A new method of sampling blood for measurement of plasma adenosine.
A new method of sampling blood for measurement of plasma adenosine.
复制标题
一种用于测量血浆腺苷的采血新方法。
DOI：
10.1152/ajpheart.1990.258.4.h1232
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Belardinelli,L
中科院分区：
文献类型：
--
作者：
Shryock,JC;Boykin,MT;Hill,JA;Belardinelli,L
37℃时，人体血液中腺苷的半衰期为1 ~ 2 s。为了准确测量血浆中腺苷的浓度，需要抑制血液通过采样导管运输过程中腺苷的代谢。为此，我们测试了一种双腔导管和一种抑制腺苷代谢的化合物溶液（停止溶液）。停止液和放射性标记腺苷通过内腔输送，在导管尖端与血液混合，并将其抽出到外腔的收集注射器中。细胞外标记物回收率为89 +/- 5%，与加入血液的标记物数量成正比。相比之下，当血液和放射性标记物通过导管后才在收集注射器中与停止液混合时，放射性标记物的回收率为7 +/- 3%。在血液通过导管的过程中，抑制腺苷形成和降解或摄取途径的重要性被证明。结果表明，采用双腔导管和停液采集人血测定血浆腺苷浓度是必要的，也是合适的。
The half-life of adenosine in human blood is 1-2 s at 37 degrees C. To measure plasma adenosine concentration accurately, it is necessary to inhibit adenosine metabolism during transit of blood through the sampling catheter. We have tested a double-lumen catheter and a solution of compounds that inhibit adenosine metabolism (stop solution) for this purpose. Stop solution and radiolabeled adenosine were delivered via an inner lumen, mixed with blood at the catheter tip, and withdrawn to a collection syringe in an outer lumen. Extracellular recovery of label was 89 +/- 5% and proportional to the quantity of label added to blood. In contrast, when blood and radiolabel were mixed with stop solution in the collecting syringe only after passage through the catheter, recovery of radiolabel was 7 +/- 3%. The importance of inhibiting pathways of adenosine formation and degradation or uptake during transit of blood through the catheter was demonstrated. The results suggest that a double-lumen catheter and stop solution are both necessary and suitable for collection of human blood to determine the concentration of plasma adenosine.