Sulfation of LH does not affect intracellular trafficking.
Sulfation of LH does not affect intracellular trafficking.
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LH 的硫酸化不影响细胞内运输。
DOI：
10.1016/j.mce.2009.03.004
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发表时间：
2009
影响因子：
4.1
通讯作者：
Boime,Irving
中科院分区：
文献类型：
--
作者：
Pearl,ChristopherA;Boime,Irving
LH和FSH由垂体前叶的相同促性腺细胞产生，但分泌方式不同。LH的分泌主要是间歇性的或受调节的，而FSH的分泌主要是基础性的或组成性的。LH和FSH的天冬酰胺（N）连接寡糖分别以硫酸盐和唾液酸终止。TSH还含有硫酸化N-连接寡糖，并通过调节途径分泌。据推测，硫酸盐在分离LH到调节途径中起作用。使用小鼠垂体模型，我们测试了这一假设，通过检查分泌的命运，从垂体处理氯酸钠，一种已知的硫酸化抑制剂。在这里，我们表明，小鼠LH是硫酸化和分泌通过调节途径，而FSH分泌组成。从氯酸盐处理的垂体，这表明硫酸化的完全抑制，LH分泌是类似于未经处理的垂体。这些数据表明，硫酸化N-连接寡糖的代谢作用不是细胞内运输，而是LH的细胞外生物活性。
LH and FSH are produced by the same gonadotrope cells of the anterior pituitary but differ in their mode of secretion. LH secretion is primarily episodic, or regulated, while FSH secretion is primarily basal, or constitutive. The asparagine (N)-linked oligosaccharides of LH and FSH terminate with sulfate and sialic acid, respectively. TSH also contains sulfated N-linked oligosaccharides and is secreted through the regulated pathway. It has been hypothesized that sulfate plays a role in segregating LH to the regulated pathway. Using a mouse pituitary model, we tested this hypothesis by examining the secretory fate of LH from pituitaries treated with sodium chlorate, a known inhibitor of sulfation. Here we show that mouse LH is sulfated and secreted through the regulated pathway, while FSH is secreted constitutively. LH secretion from chlorate-treated pituitaries, which showed complete inhibition of sulfation, was similar to untreated pituitaries. These data suggest that the metabolic role for sulfated N-linked oligosaccharides is not for intracellular trafficking but for the extracellular bioactivity of LH.