The Drosophila immune defense against gram-negative infection requires the death protein dFADD
The Drosophila immune defense against gram-negative infection requires the death protein dFADD
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DOI：
10.1016/s1074-7613(02)00454-5
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发表时间：
2002-11-01
期刊：
影响因子：
32.4
通讯作者：
Reichhart, JM
中科院分区：
文献类型：
--
作者：
Naitza, S;Rossé, C;Reichhart, JM
果蝇对革兰氏阴性感染的反应是通过依赖于IMD途径的组成部分的免疫反应来实现的。我们最近发现，IMD编码的蛋白具有与哺乳动物RIP高度相似的死亡结构域。通过酵母双杂交筛选，我们已经分离出死亡蛋白dFADD作为与IMD相关的分子。我们的数据表明，dFADD功能的丧失使苍蝇对革兰氏阴性菌感染高度敏感，而不影响对革兰氏阳性细菌的耐药性。通过遗传分析，我们发现dFADD在控制抗菌肽基因诱导性的途径中作用于IMD下游。
Drosophila responds to Gram-negative infections by mounting an immune response that depends on components of the IMD pathway. We recently showed that imd encodes a protein with a death domain with high similarity to that of mammalian RIP. Using a two-hybrid screen in yeast, we have isolated the death protein dFADD as a molecule that associates with IMD. Our data show that loss of dFADD function renders flies highly susceptible to Gram-negative infections without affecting resistance to Gram-positive bacteria. By genetic analysis we show that dFADD acts downstream of IMD in the pathway that controls inducibility of the antibacterial peptide genes.