Efficient colonization and harpins mediated enhancement in growth and biocontrol of wilt disease in tomato by Bacillus subtilis
Efficient colonization and harpins mediated enhancement in growth and biocontrol of wilt disease in tomato by Bacillus subtilis
复制标题
枯草芽孢杆菌高效定植和 harpins 介导的番茄枯萎病生长增强和生物防治
DOI：
10.1111/lam.12144
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发表时间：
2013-12-01
影响因子：
2.4
通讯作者：
Gao, X.
中科院分区：
文献类型：
--
作者：
Gao, S.;Wu, H.;Gao, X.
枯草芽孢杆菌和harpin都刺激植物生长和防御各种植物病原体。在本研究中，B.枯草杆菌168和两个衍生物，表面活性素生产者OKB 105和组合的表面活性素和HpaGXooc生产者OKBHF，施加到番茄植物，以研究这种效果的机制。为了评价定殖能力，用绿色荧光蛋白（GFP）标记菌株。虽然三种菌株在根表面的生物膜分布相似，但两种表面活性素生产者的定殖种群比菌株168高约2至3倍，并且这伴随着番茄生长的显著增加。这些结果表明，有效的定殖，可能是促进表面活性素的生产，提高了植物生长促进的效率由B。枯草杆菌。三个都是B。枯草芽孢杆菌处理导致植物在接种青枯雷尔氏菌后具有不太严重的病害症状，用OKBHF处理的植物是最抗性的，这表明hpaGXooc提高了B的生物防治效率。枯草杆菌。防御相关基因的分析显示HpaGXooc对B具有协同作用。枯草杆菌增强致病相关基因PR 1b 1和PR-P2的表达。相反，防御相关基因PINI和PINII的表达受到抑制。
Both Bacillus subtilis and harpins stimulate plant growth and defence against various plant pathogens. In this study, B. subtilis 168 and two derivatives, surfactin producer OKB105 and combined surfactin and HpaGXooc producer OKBHF, were applied to tomato plants to investigate the mechanisms underlying this effect. To evaluate colonization ability, strains were labelled with green fluorescent protein (GFP). Although biofilm distribution of the three strains was similar on root surfaces, Colonization populations of the two surfactin producers were approximately 2‐ to 3‐fold higher than that of strain 168, and this was accompanied by significantly increased tomato growth. These results suggest that efficient colonization, possibly facilitated by surfactin production, enhanced the efficiency of plant growth promotion by B. subtilis. All three B. subtilis treatments caused plants to have less severe disease symptoms after inoculation with Ralstonia solanacearum, with plants treated with OKBHF being the most resistant, suggesting that hpaGXooc improves biocontrol efficiency of B. subtilis. Analysis of defence‐related genes showed a synergistic effect of HpaGXooc on B. subtilis enhancement of the expression of the pathogenesis‐related genes PR1b1 and PR‐P2. In contrast, expression of the defence‐related genes PINI and PINII was suppressed.