A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis.
A sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis.
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DOI：
10.1007/978-1-62703-386-2_17
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Mayr, Manuel
中科院分区：
文献类型：
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作者：
Barallobre-Barreiro, Javier;Didangelos, Athanasios;Mayr, Manuel
心肌纤维化以细胞外基质(ECM)过度沉积为特征，是多种心血管疾病的常见并发症。然而，对心脏细胞外间隙中的蛋白质知之甚少。心脏ECM的蛋白质组学分析可能是具有挑战性的，因为存在更丰富的细胞内蛋白质，完整的ECM蛋白质的溶解度低，以及存在大量的翻译后修饰。在这里，我们描述了一种基于组织去细胞的提取方法，它允许在心脏组织中对细胞外蛋白进行生化细分。这些相对较低复杂性的蛋白质组分适合于凝胶-LC-MS/MS和其他蛋白质组学技术的分析。
Cardiac fibrosis is characterized by excessive deposition of extracellular matrix (ECM) and is a common complication of various cardiovascular diseases. However, little is known about proteins in the cardiac extracellular space. Proteomics analysis of cardiac ECM can be challenging due to the presence of more abundant intracellular proteins, the low degree of solubility of integral ECM proteins, and the presence of abundant posttranslational modifications. Here we describe an extraction methodology based on tissue decellularization, which allows the biochemical subfractionation of extracellular proteins in cardiac tissue. These relatively low-complexity protein fractions are suitable for analysis by gel-LC-MS/MS and other proteomics techniques.