Selection for high oridonin yield in the Chinese medicinal plant Isodon (Lamiaceae) using a combined phylogenetics and population genetics approach.
Selection for high oridonin yield in the Chinese medicinal plant Isodon (Lamiaceae) using a combined phylogenetics and population genetics approach.
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DOI：
10.1371/journal.pone.0050753
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Clardy J
中科院分区：
文献类型：
--
作者：
Harris ES;Cao S;Schoville SD;Dong C;Wang W;Jian Z;Zhao Z;Eisenberg DM;Clardy J
冬凌草甲素是一种具有抗癌活性的二萜类化合物，存在于中药植物冬凌草及一些相关物种中。虽然冬凌草甲素的生物活性已得到充分研究，但对该化合物生产过程中自然变异的程度却知之甚少。本研究描述了冬凌草甲素生产的自然变异，以指导选择冬凌草甲素产量最高的冬凌草种群。在中国采集冬凌草不同种群及相关物种，并在温室中培育其后代。检查样品的冬凌草甲素含量，使用 11 个微卫星进行基因分型，并对代表的三个系统发育标记（ITS、rps16、trnL-trnF）进行测序。使用来自每个种群的样本以及之前公布的 Genbank 序列，将冬凌草甲素的产量绘制在冬凌草属的分子系统发育图上。据报道，在检查二萜类化合物的 74 种冬凌草属植物中，有 12 种含有冬凌草甲素，系统发育表明冬凌草甲素的产生在该属中至少出现过 3 次。尽管有证据表明冬凌草甲素生产群体和非冬凌草甲素生产群体之间存在基因流动，但野生收集的亲本和温室生长的后代之间的冬凌草甲素产量却出人意料地一致。此外，个体之间的微卫星遗传距离与父母和后代的化学距离显着相关。当比较仅限于冬凌草种群时，遗传力和与遗传距离的相关性均不显着，但应使用其他样本来证实这一结果。基于这些结果，未来对冬凌草甲素产量进行冬凌草种群筛选时，首先应优先考虑对已知产生冬凌草甲素的所有物种进行广泛调查，而不是集中于一个物种的多个种群，例如冬凌草。在此检测的样品中，来自河南省的冬凌草或冬凌草是冬凌草甲素的最佳来源。
Oridonin is a diterpenoid with anti-cancer activity that occurs in the Chinese medicinal plant Isodon rubescens and some related species. While the bioactivity of oridonin has been well studied, the extent of natural variation in the production of this compound is poorly known. This study characterizes natural variation in oridonin production in order to guide selection of populations of Isodon with highest oridonin yield. Different populations of I. rubescens and related species were collected in China, and their offspring were grown in a greenhouse. Samples were examined for oridonin content, genotyped using 11 microsatellites, and representatives were sequenced for three phylogenetic markers (ITS, rps16, trnL-trnF). Oridonin production was mapped on a molecular phylogeny of the genus Isodon using samples from each population as well as previously published Genbank sequences. Oridonin has been reported in 12 out of 74 species of Isodon examined for diterpenoids, and the phylogeny indicates that oridonin production has arisen at least three times in the genus. Oridonin production was surprisingly consistent between wild-collected parents and greenhouse-grown offspring, despite evidence of gene flow between oridonin-producing and non-producing populations of Isodon. Additionally, microsatellite genetic distance between individuals was significantly correlated with chemical distance in both parents and offspring. Neither heritability nor correlation with genetic distance were significant when the comparison was restricted to only populations of I. rubescens, but this result should be corroborated using additional samples. Based on these results, future screening of Isodon populations for oridonin yield should initially prioritize a broad survey of all species known to produce oridonin, rather than focusing on multiple populations of one species, such as I. rubescens. Of the samples examined here, I. rubescens or I. japonicus from Henan province would provide the best source of oridonin.