Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood
Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood
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基于酰化衍生化的 LC-MS 分析指尖血中 25-羟基维生素 D
DOI：
10.1194/jlr.d092197
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发表时间：
2019-05-01
影响因子：
6.5
通讯作者：
Zhang, Bing-Hong
中科院分区：
文献类型：
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作者：
Le, Juan;Yuan, Teng-Fei;Zhang, Bing-Hong
维生素D代谢产物分析对儿科具有重要的临床应用价值。然而，侵入性静脉穿刺采样和高血液消耗给患者带来了许多痛苦。为了缓解，我们进行了LC-MS方法25-羟基维生素D的定量只有3升的血浆从相当少的侵入性手指采血样品。为了提高灵敏度，在维生素D代谢物衍生化中首次引入了对C3-羟基的酰化（通过异烟酰氯）而不是对s-顺式-二烯结构的Diels-Alder加合。与现有的衍生化方法相比，这种新型策略不仅可以防止异构体干扰，而且具有更高的反应通量。对于认证，该方法进行了系统验证，并显示出令人满意的一致性与SRM 927 a。在临床应用中，我们发现黄疸新生儿25-羟基维生素D和间接/总胆红素之间有令人信服的相关性。这样的观察表明，维生素D补充剂可能有助于实现新生儿黄疸的最佳结果。
Vitamin D metabolite analysis possessed significant clinical value for the pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3 l of plasma from the considerably less invasive finger-prick blood samples. To improve sensitivity, acylation on C3-hydroxyl (by isonicotinoyl chloride) rather than Diels-Alder adduction on s-cis-diene structure was for the very first time introduced into vitamin D metabolite derivatization. Compared with the existing derivatization approaches, this novel strategy not only prevented isomer interference, but also exhibited higher reacting throughput. For certification, the methodology was systematically validated and showed satisfying consistency with SRM927a. During clinical application, we found a convincing correlation between 25-hydroxyvitamin D and indirect/total bilirubin in jaundiced newborns. Such an observation indicated that vitamin D supplementation may help to achieve optimal outcomes in neonatal jaundice.