Inhibition of the replication of native and 3'-azido-2',3'-dideoxythymidine (AZT)-resistant simian immunodeficiency virus (SIV) by 3-nitrosobenzamide.
Inhibition of the replication of native and 3'-azido-2',3'-dideoxythymidine (AZT)-resistant simian immunodeficiency virus (SIV) by 3-nitrosobenzamide.
复制标题
3-亚硝基苯甲酰胺抑制天然和 3-叠氮基-2,3-二脱氧胸苷 (AZT) 抗性猿猴免疫缺陷病毒 (SIV) 的复制。
DOI：
10.1016/0014-5793(93)81778-x
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Kun,E
中科院分区：
文献类型：
--
作者：
Chuang,AJ;KillamJr,KF;Chuang,RY;Rice,WG;Schaeffer,CA;Mendeleyev,J;Kun,E
当病毒感染前将CEM × 174细胞与3-亚硝基苯甲酰胺（NOBA）药物预孵育1 h时，20 μM浓度的NOBA药物可显著消除SIV复制。用20 μM NOBA处理CEM × 174细胞，PCR检测发现，Gag基因的DNA合成受到抑制。细胞活力与NOBA的抗病毒作用成正比。在体外，N 0 BA以浓度依赖性方式抑制AZT抗性SIV 23740在MMU 23740细胞中的复制，而对细胞活力没有显著影响。SIVmac 239的逆转录酶活性不受高达800 μM浓度的NOBA的影响。用NOBA预孵育两株SIV完全消除了它们在人PHA-PBL细胞中的感染性。NOBA对两株SIV在PHA-PBL细胞中的复制也有抑制作用。
The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 μM concentration when CEM × 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM × 174 cells with 20 μM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for thegaggene, as determined by the PCR technique. Cell viability was directly proportional to the antiviral action of NOBA. Replication of AZT-resistant SIV 23740 in MMU 23740 cells in vitro was suppressed by NOBA in a concentration-dependent manner without significant effects on cell viability. Reverse transcriptase activity of SIVmac239 was unaffected by NOBA up to 800 μM concentration. Preincubation of two SIV strains with NOBA completely abolished their infectivity in human PHA-PBL cells. Replication of two strains of SIV in PHA-PBL cells was also inhibited by NOBA.