Oral tolerance in humans. T cell but not B cell tolerance after antigen feeding.
Oral tolerance in humans. T cell but not B cell tolerance after antigen feeding.
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DOI：
10.4049/jimmunol.152.9.4663
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发表时间：
1994-05
影响因子：
4.4
通讯作者：
Steffen Husby;Jiri Mestecky;Z. Moldoveanu;S. Holland;C. Elson
中科院分区：
文献类型：
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作者：
Steffen Husby;Jiri Mestecky;Z. Moldoveanu;S. Holland;C. Elson
本研究的目的是调查口服耐受，定义为银喂养后，银特异性免疫无反应，是否可以诱导人类长期摄入银。8名成年志愿者摄入总剂量为0.5 g的匙孔血蓝蛋白（KLH），然后皮下免疫接种KLH。8名对照仅接受皮下免疫。在喂食KLH的组中，KLH特异性T细胞增殖显著减少（p = 0.04），皮肤试验对KLH的反应延迟（p = 0.07）。单独摄入KLH不会在血清或分泌物中诱导显著水平的抗体。然而，在随后的皮下免疫后，循环IgG和IgM抗KLH产生细胞的数量，血清IgG，伊加和IgM抗KLH抗体的滴度，以及唾液和肠分泌物中伊加抗KLH抗体的滴度在KLH喂养组中显著高于非喂养组。我们的结论是，KLH喂养诱导系统性T细胞耐受，但B细胞引发，在系统和粘膜网站。这些研究支持使用Ag喂养作为某些免疫介导疾病的治疗的概念。
The purpose of this study was to investigate whether oral tolerance, defined as Ag-specific immunologic unresponsiveness after Ag feeding, could be induced in humans after prolonged Ag ingestion. Eight adult volunteers ingested a total dose of 0.5 g of keyhole limpet hemocyanin (KLH) followed by subcutaneous immunization with KLH. Eight controls received only the subcutaneous immunization. In the group fed KLH, there was a significant reduction in KLH-specific T cell proliferation (p = 0.04) and delayed skin test responses (p = 0.07) to KLH. KLH ingestion alone did not induce significant levels of Abs in either serum or secretions. However, after the subsequent subcutaneous immunization, the number of circulating IgG and IgM anti-KLH-producing cells, the titers of serum IgG, IgA, and IgM anti-KLH Abs, and the titers of IgA anti-KLH Abs in saliva and intestinal secretions were significantly greater in the KLH-fed group than in the nonfed group. We conclude that KLH feeding induced systemic T cell tolerance, but B cell priming, at both systemic and mucosal sites. These studies support the concept of using Ag feeding as a treatment for certain immune-mediated diseases.