Tissue engineering of perfused microvessels
Tissue engineering of perfused microvessels
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DOI：
10.1016/s0026-2862(03)00040-2
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发表时间：
2003-07-01
影响因子：
3.1
通讯作者：
Sanders, JE
中科院分区：
文献类型：
--
作者：
Neumann, T;Nicholson, BS;Sanders, JE
创建和生存更大的三维组织的一个主要障碍是缺乏提供氧气，营养物质和代谢副产物运输的血管网络。尽管先前已经描述了在体外产生微脉管系统的尝试（Microcirculation 2（1995），377; Tissue Eng.6（2000），105; Ann. NY Accd. Sci. 944（2001），443），这些方法依赖于组织构建体内空隙空间的血管化或宿主血管对空毛细血管网络的利用。在本研究中，我们研究了在体外建立灌注微血管的可能性，这些微血管可以包含在人工组织中。首先，将末端与微管配合的尼龙线股定位在小灌注室中。然后将血管平滑肌细胞（SMC）接种到尼龙线和管上。细胞增殖形成同心层。培养21天后，层厚度约为100 μ m，培养28天后为150 μ m。然后将管路拔出，并将腔室连接到灌注系统。用培养基连续灌注血管超过7天而没有失败。人工微血管在组织工程学和血管研究中可能是有用的模型。(C)2003 Elsevier Science（美国）。All rights reserved.
One major obstacle toward the creation and survival of larger, three-dimensional tissues is the lack of a vascular network that provides transport of oxygen, nutrients, and metabolic byproducts. Although attempts to create microvasculature in vitro have been described previously (Microcirculation 2 (1995), 377; Tissue Eng. 6 (2000), 105; Ann. NY Accd. Sci. 944 (2001), 443), these methods depend on vascularization of void spaces within the tissue-construct or on the utilization of empty capillary networks by host vessels. In the present study, we examined the possibility of creating perfused microvessels in vitro that can be included in an artificial tissue. First, strands of nylon line with their ends fit into microtubing were positioned within small perfusion chambers. Vascular smooth muscle cells (SMCs) were then seeded onto the nylon strands and tubing. The cells multiplied to form concentric layers. Layer thickness was approximately 100 mum after 21 days and 150 mum after 28 days of culture. The lines were then extracted and the chambers connected to a perfusion system. The vessels were continuously perfused with culture medium over 7 days without failure. Artificial microvessels may prove useful in tissue engineering and as models for vascular research. (C) 2003 Elsevier Science (USA). All rights reserved.