Effect of peritoneal fluid from endometriosis patients on endometrial stromal cell proliferation in vitro.
Effect of peritoneal fluid from endometriosis patients on endometrial stromal cell proliferation in vitro.
复制标题
子宫内膜异位症患者腹腔液对子宫内膜基质细胞体外增殖的影响。
DOI：
10.1097/00006250-199011000-00013
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发表时间：
1990
影响因子：
7.2
通讯作者：
Halme,J
中科院分区：
文献类型：
--
作者：
Surrey,ES;Halme,J
在短期培养中生长的晚期增殖期子宫内膜基质细胞被用作子宫内膜异位植入物基质成分的模型。细胞在单独的培养基和补充有5%、10%和20%浓度的从患有或不患有子宫内膜异位症的患者腹膜液中获得的无细胞部分的培养基中生长。根据腹腔镜检查时是否存在子宫内膜异位症以及腹膜液雌二醇浓度的相似性，对九对液体样本进行匹配。暴露于子宫内膜异位症腹膜液的细胞比暴露于非子宫内膜异位症腹膜液的细胞在72小时内的连续细胞收获过程中通过3 H-胸苷掺入反映的基质细胞增殖更大。当暴露于浓度为 10% 和 20% 的腹膜液中时，这达到了统计学显着性（P<.05）。 3 H-胸苷掺入和腹膜液浓度之间的线性剂量反应关系只能针对暴露于从子宫内膜异位症患者获得的液体样品的基质细胞得出(r=0.51；P<.001)。此外，在 20% 子宫内膜异位症腹膜液中生长的细胞在 72 小时内的增殖显着高于仅在营养培养基中生长的细胞 (P<.001)。这些数据表明，分泌到子宫内膜异位症患者腹膜液中的因子可能在疾病植入物的增殖或维持中发挥作用。(Obstet Gynecol 76: 792, 1990)
Late proliferative phase endometrial stromal cells grown in short-term culture were used as a model for the stromal component of endometriotic implants. Cells were grown in medium alone and in medium supplemented by 5, 10, and 20% concentrations of the cell-free fractions of peritoneal fluid obtained from patients with and without endometriosis. Nine fluid-sample pairs were matched based on the presence or absence of endometriosis at laparoscopy and the similarity of peritoneal fluid estradiol concentrations. Stromal cell proliferation as reflected by 3 H-thymidine incorporation during sequential cell harvests over 72 hours was greater for cells exposed to endometriosis peritoneal fluid than for those exposed to non-endometriosis peritoneal fluid. This reached statistical significance with exposure to peritoneal fluid concentrations of 10 and 20%(P<. 05). A linear dose-response relationship between 3 H-thymidine incorporation and peritoneal fluid concentration could be derived only for stromal cells exposed to fluid samples obtained from endometriosis patients (r= 0.51; P<. 001). In addition, proliferation over 72 hours was significantly greater for cells grown in 20% endometriosis peritoneal fluid than for those grown in nutrient medium alone (P<. 001). These data imply that factor (s) secreted into the peritoneal fluid of endometriosis patients may play a role in the proliferation or maintenance of disease implants.(Obstet Gynecol 76: 792, 1990)