Comparative transcriptome profiling of near-isogenic barley (Hordeum vulgare) lines differing in the allelic state of a major grain protein content locus identifies genes with possible roles in leaf senescence and nitrogen reallocation
Comparative transcriptome profiling of near-isogenic barley (Hordeum vulgare) lines differing in the allelic state of a major grain protein content locus identifies genes with possible roles in leaf senescence and nitrogen reallocation
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DOI：
10.1111/j.1469-8137.2007.02270.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Fischer, Andreas M.
中科院分区：
文献类型：
--
作者：
Jukanti, Aravind K.;Heidlebaugh, Nancy M.;Fischer, Andreas M.
为了鉴定参与叶片衰老和全株氮重新分配的调控和执行的基因，比较了在衰老时间和成熟籽粒蛋白质浓度方面存在差异的近等基因大麦种质。经过四代回交后，获得了在控制籽粒蛋白质浓度的第六号染色体上的主要基因座上等位基因状态不同的大麦品系。根据表明开花后 14-21 天低粒蛋白种质和高粒蛋白种质之间存在主要差异的生理数据，使用 22-k Barley1 Affymetrix 微阵列对这些时间点的低蛋白亲本和一个高蛋白近等基因系的旗叶和籽粒转录组进行了比较。我们的数据将多个具有已知（基于序列比较）和未知功能的基因与衰老过程相关联。其中包括富含亮氨酸的重复跨膜蛋白激酶、与 AtGRP7 同源的富含甘氨酸的 RNA 结合蛋白和“FT/TF1 之母”基因。我们的数据还表明，种质中质体和质体外蛋白酶编码基因的上调会加速叶片衰老。本研究确定的候选基因的功能特征可能有助于我们了解叶片衰老和氮重新分配背后的分子网络。
To identify genes involved in the regulation and execution of leaf senescence and whole-plant nitrogen reallocation, near-isogenic barley germplasm divergent in senescence timing and protein concentration of mature grains was contrasted.Barley lines differing in allelic state at a major locus on chromosome six, controlling grain protein concentration, were obtained after four generations of backcrossing. Based on physiological data indicating major differences between low- and high-grain protein germplasm at 14-21 d past anthesis, the flag leaf and kernel transcriptomes of the low-protein parent and one high-protein near-isogenic line were compared at these time points, using the 22-k Barley1 Affymetrix microarray.Our data associate several genes with both known (based on sequence comparisons) and unknown functions with the senescence process. These include leucine-rich repeat transmembrane protein kinases, a glycine-rich RNA-binding protein with homology to AtGRP7 and a 'mother of FT/TF1' gene. Our data also indicate upregulation of genes coding for both plastidial and extraplastidial proteases in germplasm with accelerated leaf senescence.Functional characterization of candidate genes identified by this research may contribute to our understanding of the molecular network underlying leaf senescence and nitrogen reallocation.