Deletion hot spots in chimeric Escherichia coli plasmids
Deletion hot spots in chimeric Escherichia coli plasmids
复制标题
嵌合大肠杆菌质粒中的缺失热点
DOI：
10.1128/jb.171.4.1846-1853.1989
复制
发表时间：
1989
影响因子：
3.2
通讯作者：
S. Ehrlich
中科院分区：
文献类型：
--
作者：
B. Michel;E. d’Alençon;S. Ehrlich
在由M13 mp2、pBR 322和pC194组成的嵌合质粒中经常形成缺失（B. Michel和S. D.埃利希，美国国家科学院院刊。Acad. sci. USA 83：3386 - 3390，1986）。它们通过将M13复制起点中切口位点附近的核苷酸连接到不相邻的核苷酸而产生。这种核苷酸通常位于特定的短质粒区域内，称为缺失热点。嵌合质粒中存在三个天然热点。两个是活跃的，只有当开始在M13起点的DNA复制被允许进行;第三个是活跃的，只有在存在野生型量的DNA连接酶。通过在质粒中产生回文序列产生三个人工热点。
Deletions form frequently in chimeric plasmids composed of M13mp2, pBR322, and pC194 (B. Michel and S. D. Ehrlich, Proc. Natl. Acad. Sci. USA 83:3386-3390, 1986). They are generated by joining of the nucleotide neighboring the nick site in the M13 replication origin to a nonadjacent nucleotide. This nucleotide is most often located within particular short plasmid regions, named deletion hot spots. Three natural hot spots were present in the chimeric plasmids. Two were active only when the DNA replication initiated at the M13 origin was allowed to progress; the third was active only in the presence of wild-type amounts of DNA ligase. Three artificial hot spots were generated by creating palindromic sequences in the plasmids.
回文和非回文序列在体外和体内阻止 DNA 合成中的作用。
影响因子：
5.6
作者：
Weaver,DT;DePamphilis,ML
通讯作者：
DePamphilis,ML
影响因子：
2.9
作者：
LADUCA, RJ;FAY, PJ;BAMBARA, RA
通讯作者：
BAMBARA, RA
DOI：
10.1073/pnas.79.4.983
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KAGUNI, LS;CLAYTON, DA
通讯作者：
CLAYTON, DA