High resolution electrophoretic variation at the esterase-6 locus in a natural population of Drosophila melanogaster
High resolution electrophoretic variation at the esterase-6 locus in a natural population of Drosophila melanogaster
复制标题
果蝇自然群体中酯酶 6 位点的高分辨率电泳变异
DOI：
10.1038/hdy.1987.121
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发表时间：
1987
期刊：
影响因子：
3.8
通讯作者：
J. Oakeshott
中科院分区：
文献类型：
--
作者：
P. Cooke;R. Richmond;J. Oakeshott
从黑腹果蝇的天然种群中提取了 157 个酯酶 6 等位基因系。通过在高分辨率醋酸纤维素板上重复成对比较来确定不同品系中 Est6 同种异型酶的相对电泳迁移率。在品系中解析出十种异酶类别，其中只有四种，Est6-VF、Est6-F、Est6-F和Est6-S，使用标准电泳程序是明显的。额外的变化是由于 Est6-F 和 Est6-S 分别细分为三类和五类而产生的。遗传分析证实，额外的类别映射在 Est6 基因座处或附近。群体频率较低的另外两个类别与显着较高的第三染色体活力相关。 Est6-F 中最常见的类别与 In(3L)P 相关的多态性倒位处于显着的配子不平衡状态。将相同的高分辨率程序应用于代表 6 个 Est6 热稳定性变体的 13 个黑腹果蝇品系。不同的热稳定性变体并没有根据电泳类别进行一致的分组。这意味着这两个标准彼此独立地检测蛋白质变异，并且发生的变异比单独使用任一标准检测到的变异大得多。
One hundred and fifty-seven lines isoallelic for esterase-6 were extracted from a natural population of D. melanogaster. The relative electrophoretic mobilities of Est6 allozymes in the different lines were determined by repeated pair-wise comparisons on high resolution cellulose acetate plates. Ten allozymic classes were resolved among the lines where only four, Est6-VF, Est6-F' Est6-F and Est6-S, had been evident using standard electrophoretic procedures. The additional variation resulted from the subdivision of Est6-F and Est6-S into three and five classes respectively. Genetic analyses confirmed that the additional classes mapped at or near the Est6 locus. Two of the additional classes occurring at low population frequencies were associated with significantly higher third chromosome viabilities. The most frequent class within Est6-F was in significant gametic disequilibrium with the linked polymorphic inversion In(3L)P. The same high resolution procedures were applied to 13 lines of D.melanogaster representing six Est6 thermostability variants. Different thermostability variants did not group consistently according to electrophoretic classes. This implies that the two criteria are detecting protein variation independently from each other and that much greater variation occurs than is detected by either criterion alone.