Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution
Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution
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DOI：
10.1126/science.1108625
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发表时间：
2005-05-20
期刊：
影响因子：
56.9
通讯作者：
Gingeras, TR
中科院分区：
文献类型：
--
作者：
Cheng, J;Kapranov, P;Gingeras, TR
在八种细胞系中以5碱基对分辨率映射了10个人类染色体的聚腺苷酸化和非多聚腺苷酸化RNA的转录位点。未经通知的非质腺苷化转录本构成了人类基因组转录输出的主要比例。在所有转录的序列中，分别观察到分别是多核，非多烯基化和双态的，分别为19.4、43.7和36.9％。所有转录序列的一半仅在细胞核中发现，并且在大多数情况下都没有被没收。总体而言，人类基因组的转录部分主要由poly a+和poly a-注释的转录本的交织网络和未知功能的未注释的转录本组成。该组织对解释基因型 - 表型关联，基因表达的调节和基因的定义具有重要意义。
Sites of transcription of polyadenylated and nonpolyadenylated RNAs for 10 human chromosomes were mapped at 5-base pair resolution in eight cell lines. Unannotated, nonpotyadenylated transcripts comprise the major proportion of the transcriptional output of the human genome. Of all transcribed sequences, 19.4, 43.7, and 36.9% were observed to be polyadenytated, nonpolyadenylated, and bimorphic, respectively. Half of all transcribed sequences are found only in the nucleus and for the most part are unannotated. Overall, the transcribed portions of the human genome are predominantly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcripts of unknown function. This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene.