Acute heat stress up-regulates neuropeptide Y precursor mRNA expression and alters brain and plasma concentrations of free amino acids in chicks
Acute heat stress up-regulates neuropeptide Y precursor mRNA expression and alters brain and plasma concentrations of free amino acids in chicks
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DOI：
10.1016/j.cbpa.2015.04.010
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发表时间：
2015-09-01
影响因子：
2.3
通讯作者：
Chowdhury, Vishwajit S.
中科院分区：
文献类型：
--
作者：
Ito, Kentaro;Bahry, Mohammad A.;Chowdhury, Vishwajit S.
热应激导致鸡的体温升高和摄食量减少。几种神经肽和氨基酸在调节食物摄入方面起着至关重要的作用。然而，神经肽和氨基酸对热应激诱导的摄食调节的反应知之甚少。在目前的研究中，下丘脑的一些神经肽的mRNA表达相关的食物摄入量和脑和血浆中的游离氨基酸的含量进行了检查，在14日龄的小鸡暴露于高环境温度（HT; 40 +/- 1摄氏度2或5小时）或控制热中性温度（CT; 30 +/- 1摄氏度）。HT显着增加直肠温度和血浆皮质酮水平和抑制食物摄入。HT还增加了神经肽Y（NPY）和刺鼠信号蛋白（ASIP）前体mRNA的表达，而阿黑皮素原、胆囊收缩素、生长激素释放肽或促肾上腺皮质激素释放激素前体mRNA的表达无变化。进一步发现，与CT鸡相比，HT鸡的间脑游离氨基酸含量-即色氨酸、亮氨酸、异亮氨酸、缬氨酸和丝氨酸-显著更高，其血浆氨基酸有一些变化。HT时NPY和ASIP表达的诱导以及某些游离氨基酸的变化提示这些变化可能是摄食抑制的结果或原因。(C)© 2015 Elsevier Inc版权所有。
Heat stress causes an increase in body temperature and reduced food intake in chickens. Several neuropeptides and amino acids play a vital role in the regulation of food intake. However, the responses of neuropeptides and amino acids to heat-stress-induced food-intake regulation are poorly understood. In the current study, the hypothalamic mRNA expression of some neuropeptides related to food intake and the content of free amino acids in the brain and plasma was examined in 14-day-old chicks exposed to a high ambient temperature (HT; 40 +/- 1 degrees C for 2 or 5 h) or to a control thermoneutral temperature (CT; 30 +/- 1 degrees C). HT significantly increased rectal temperature and plasma corticosterone level and suppressed food intake. HT also increased the expression of neuropeptide Y (NPY) and agouti-signaling protein (ASIP) precursor mRNA, while no change was observed in pro-opiomelanocortin, cholecystokinin, ghrelin, or corticotropin-releasing hormone precursor mRNA. It was further found that the diencephalic content of free amino acids - namely, tryptophan, leucine, isoleucine, valine and serine - was significantly higher in HT chicks with some alterations in their plasma amino acids in comparison with CT chicks. The induction of NPY and ASIP expression and the alteration of some free amino acids during HT suggest that these changes can be the results or causes the suppression of food intake. (C) 2015 Elsevier Inc All rights reserved.