THE OPAQUE-2 MUTATION OF MAIZE DIFFERENTIALLY REDUCES ZEIN GENE-TRANSCRIPTION
THE OPAQUE-2 MUTATION OF MAIZE DIFFERENTIALLY REDUCES ZEIN GENE-TRANSCRIPTION
复制标题
DOI：
10.1105/tpc.1.1.105
复制
发表时间：
1989-01-01
期刊：
影响因子：
11.6
通讯作者：
LARKINS, BA
中科院分区：
文献类型：
--
作者：
KODRZYCKI, R;BOSTON, RS;LARKINS, BA
玉米蛋白是玉米种子的贮藏蛋白，由一个大的多基因家族编码，在胚乳发育过程中受发育调控并以组织特异性方式表达。这些蛋白质的合成受到突变的影响，如不透明-2，导致玉米蛋白和mrna的积累减少。我们使用核转录法分析了玉米蛋白基因在发育中的正常胚乳和不透明胚乳中的表达，并绘制了这些基因编码区和非编码区转录本的起源图。这些分析表明，玉米蛋白基因表达在发育中的胚乳中受到转录和转录后调控。α编码基因的转录。-玉米蛋白在不透明-2突变体中被显著抑制，编码Mr 22000蛋白的表达几乎完全被阻断。其他基因亚家族也受到影响，但影响程度较轻。
Zeins, the storage proteins of maize seed, are encoded by a large multigene family that is regulated developmentally and expressed in a tissue-specific manner during endosperm development. The synthesis of these proteins is affected by mutations, such as opaque-2, that cause a reduction in the accumulation of zein proteins and mRNAs. We used nuclear run-on transcription assays to analyze the expression of zein genes in developing normal and opaque-2 endosperms and to map the origin of these trnascripts with respect to the coding and noncoding regions of the genes. These analyses demonstrate that zein gene expression is regulated transcriptionally anbd posttranscriptionally in developing endosperm. Transcription of genes encoding .alpha.-zeins is inhibited significantly in opaque-2 mutants, with expression of those encoding the Mr 22,000 proteins being almost totally blocked. Other gene subfamilies were affected but to a lesser extent.