CLASSIFICATION OF MONOAMINE NEURONES IN RAT MESENCEPHALON - DISTRIBUTION OF A NEW MONOAMINE NEURONE SYSTEM
CLASSIFICATION OF MONOAMINE NEURONES IN RAT MESENCEPHALON - DISTRIBUTION OF A NEW MONOAMINE NEURONE SYSTEM
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DOI：
10.1016/0006-8993(71)90324-6
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
STENEVI, U
中科院分区：
文献类型：
--
作者：
BJORKLUND, A;FALCK, B;STENEVI, U
通过显微荧光光谱法结合各种药物治疗，对大鼠中脑中含有单胺的大型神经元系统进行了表征和分类。以往的荧光组织化学研究表明，该区域有绿色荧光和黄色荧光神经元。本研究证实了含儿茶酚胺的绿色荧光神经元的身份。黄色荧光神经元被发现是两个明显不同的类型。一种（所谓的C型）被鉴定为含有5-羟色胺（5-HT）。这种类型具有特征的5-HT光谱，在315和380-390/520-535 nm处具有激发/发射最大值，并显示出快速的光分解。利血平或色氨酸-5-羟化酶抑制剂，p-氯苯丙氨酸治疗的荧光被取消或强烈减少，并与MAO抑制剂，nialamide治疗后显着增加。第二种黄色荧光神经元（即所谓的B型）具有明显不同于5-HT神经元的特征：激发最大值在315、370-380和420 nm，发射最大值在495-510 nm，光分解速率相当慢。这种B型的黄色荧光团被废除或强烈利血平治疗后减少，但不受治疗与p-氯苯丙氨酸或nialamide。B型细胞体与中缝核内的5-HT细胞体混杂分布，大部分B型细胞体位于尾侧线形核和中缝正中核（B_8），并分散在后者外侧的翼状结构中。B-型和5-HT型纤维，可以追溯到，至少部分，中缝核部分分离的腹内侧被盖。在前面，它们进入了内侧前脑束，在那里它们混合在一起。结论：对氯苯丙氨酸抗性的B型神经元是一个形态学和组织化学上明确的独立实体。讨论了在B型神经元中发现一种迄今未知的吲哚胺的可能性。
The large monoamine-containing neurone systems in the rat mesencephalon have been characterized and classified by means of microspectrofluorometry in combination with various drug treatments. Previous fluorescence histochemical studies have demonstrated gree fluorescent and yellow fluorescent neurones in this region. The identity of the green fluorescent neurons as catecholamine-containing was confirmed in the present study. The yellow fluorescent neurones were found to be of two distinctly different types. One (the so-called C-type) was identified as 5-hydroxytryptamine (5-HT)-containing. This type had the characteristic 5-HT spectra with the excitation/emission maxima at 315 and 380–390/520–535 nm and showed a rapid photodecomposition. The fluorescence was abolished or strongly reduced by treatment with reserpine or the tryptophan-5-hydroxylase inhibitor,p-chlorophenylalanine, and was markedly increased after treatment with the MAO inhibitor, nialamide. The second yellow fluorescent neurone type (the so-called B-type) had characteristics clearly deviating from those of the 5-HT neurones: the excitation maxima were at 315, 370–380 and 420 nm, the emission maximum was at 495–510 nm, and the photodecomposition rate was rather slow. This B-type of yellow fluorophore was abolished or strongly reduced after the reserpine treatment but was not affected by the treatments withp-chloropheylalanine or nialamide. The B-type cell bodies were found intermingled with the 5-HT cell bodies in the raphe nuclei, most of the B-type cell bodies being localized in the nucleus linearis caudalis and the nucleus medianus raphe (B8) and scattered in a wing-like structure situated lateral to this latter nucleus. The B-type and the 5-HT type of fibres which could, at least partly, be traced back to the raphe nuclei were partially separated in the ventromedial tegmentum. Anteriorly, they ran into the medial forebrain bundle where they intermingled. It is concluded that thep-chlorophenylalanine-resistant B-type neurone is a morphologically and histochemically well-defined and separate entity. The possibility is discussed that a hitherto unknown indolamine is found in the B-type neurone.