Optical aggregometry of red blood cells associated with the blood-clotting reaction in extracorporeal circulation support
Optical aggregometry of red blood cells associated with the blood-clotting reaction in extracorporeal circulation support
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DOI：
10.1007/s10047-016-0895-8
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发表时间：
2016-09-01
影响因子：
1.3
通讯作者：
Maruyama, Osamu
中科院分区：
文献类型：
--
作者：
Sakota, Daisuke;Kosaka, Ryo;Maruyama, Osamu
红细胞（rbc）的聚集性与血浆蛋白（如纤维蛋白原和脂蛋白）对凝血的贡献有关。因此，我们假设红细胞聚集性反映了血液凝固反应。提出了一种无创光学监测方法来测量红细胞聚集性，以评估机械循环支持期间的凝血阶段。使用肝素化的新鲜猪血，用旋转血泵进行体外血栓形成试验。采用光纤引导波长为785 nm的近红外激光。检测入射光、前散射光和后散射光的光纤固定在内径为1/4英寸的回路管上。由于在低剪切流速下有大量的红细胞聚集，通过控制泵转速产生脉动流。在40 s的时间内，流量从0升/分钟变化到8.5升/分钟。当气流停止时，前后散射光的强度发生了巨大变化。在低流量条件下，通过流动停止时透射光强的增加率来评价聚合性。当向循环血液中加入鱼精蛋白停止抗凝时，实验开始了。红细胞聚集性的降低与纤维蛋白原和血小板数量的减少有关。利用光学测量结合旋转血泵的脉动流量控制，可以实现对血栓形成风险的连续、无创监测。红细胞聚集法是一种潜在的无标记评价凝血风险的方法。
The aggregability of red blood cell (RBCs) is associated with the contribution of plasma proteins, such as fibrinogen and lipoproteids, to blood-clotting. Hence, we hypothesized that RBC aggregability reflects the blood-clotting reaction. A noninvasive optical monitoring method to measure RBC aggregability for the assessment of blood-clotting stage during mechanical circulatory support was developed. An in vitro thrombogenic test was conducted with a rotary blood pump using heparinized fresh porcine blood. Near-infrared laser light at a wavelength of 785 nm was guided by an optical fiber. The fibers for detecting incident, forward-, and backward-scattered light were fixed on the circuit tubing with an inner diameter of 1/4 inch. Because there is substantial RBC aggregation at low shear flow rates, a pulsatile flow was generated by controlling the pump rotational speed. The flow rate was changed from 0 to 8.5 L/min at a period of 40 s. The intensities of forward- and backward-scattered light changed dramatically when the flow stopped. The aggregability was evaluated by the increase ratio of the transmitted light intensity from the flow stopping in the low-flow condition. The experiment started when the anticoagulation was stopped by the addition of protamine into the circulating blood. Reduction in RBC aggregability was associated with a decrease in the amount of fibrinogen and the number of platelets. Continuous, noninvasive monitoring of thrombosis risk is possible using optical measurements combining pulsatile flow control of a rotary blood pump. RBC aggregometry is a potential label-free method for evaluating blood-clotting risk.