Food deprivation modulates chronic stress effects on object recognition in male rats: role of monoamines and amino acids
Food deprivation modulates chronic stress effects on object recognition in male rats: role of monoamines and amino acids
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DOI：
10.1016/s0006-8993(99)01380-3
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发表时间：
1999-05-29
期刊：
影响因子：
2.9
通讯作者：
Luine, VN
中科院分区：
文献类型：
--
作者：
Beck, KD;Luine, VN
物体识别任务被用来确定慢性束缚应激(每天6小时，持续21天)是否会损害非空间记忆，因为慢性束缚被认为会损害空间记忆。此外，在这项不依赖奖励的任务中，食物剥夺被测试为任何压力效应的可能调节因素。在每日限制3周后，在两个单独的实验中，受试者在一周内接受了开阔场地活动和物体识别(在不同的延迟间隔)的测试。实验1涉及低需求条件下的测试(小竞技场)，而实验2涉及高需求条件下的测试(大竞技场)。在实验一中评估了基础单胺和氨基酸水平(在家中笼子)，在实验二中评估了单胺唤醒水平(在抽样试验之后)。我们观察到，当延迟延长超过1h时，慢性应激会损害物体识别，而食物剥夺可以减轻损害的程度。此外，慢性应激与杏仁核和海马区去甲肾上腺素水平以及前额叶皮质多巴胺(HVA/DA，DOPAC/DA)水平升高有关。这些变化在随后被剥夺食物的应激受试者中没有观察到。缺乏食物的受试者前额叶皮质和海马区的基础5-羟色胺活性较高，血清皮质醇水平较高。结果表明，食物剥夺可能是一种新的应激，从而增加受试者对物体的唤醒和注意力，这有助于应激性受试者，特别是在低需求条件下。束缚和食物剥夺都会影响到与记忆功能相关的特定边缘区域。(C)1999 Elsevier Science B.V.保留所有权利。
An object recognition task was used to determine if chronic restraint stress (6 h/day for 21 days) impairs non-spatial memory, since chronic restraint is known to impair spatial memory. In addition, food deprivation was tested as a possible modulating factor of any stress effect in this non-reward-dependent task. Following 3 weeks of daily restraint, subjects were tested for open field activity and object recognition (over different delay intervals) during one week in two separate experiments. Experiment 1 involved testing under low demand conditions (small arena) while experiment 2 involved testing under higher-demand conditions (large arena). Basal monoamine and amino acid levels (home cage) were assessed in experiment one and monoamine arousal levels (following a sample trial) were assessed in experiment two. We observed that chronic stress impaired object recognition when the delay was extended beyond 1 h, and that food deprivation could attenuate the degree of impairment. In addition, chronic stress was associated with increased norepinephrine levels in both the amygdala and hippocampus, and dopamine (HVA/DA, DOPAC/DA) in prefrontal cortex. These changes were not observed in stress subjects that were subsequently food deprived. Food deprived subjects had higher basal serotonin activity in prefrontal cortex and hippocampus as well as higher serum CORT levels. Results suggest that food deprivation may act as a novel stress, thereby increasing subjects' arousal and attention toward the objects, which aids stressed subjects, especially in low-demand conditions. Both restraint and food deprivation affected select limbic areas associated with memory functioning. (C) 1999 Elsevier Science B.V. All rights reserved.