Studies on middle silkgland proteins of cocoon colour sex-limited silkworm (Bombyx mori L.) using two-dimensional polyacrylamide gel electrophoresis
Studies on middle silkgland proteins of cocoon colour sex-limited silkworm (Bombyx mori L.) using two-dimensional polyacrylamide gel electrophoresis
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DOI：
10.1007/bf02702560
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发表时间：
2004-03
影响因子：
2.9
通讯作者：
Yuanxiang Jin;Yu-yin Chen;Meng-kui Xu;Yonghuang Jiang
中科院分区：
文献类型：
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作者：
Yuanxiang Jin;Yu-yin Chen;Meng-kui Xu;Yonghuang Jiang
采用高分辨双向聚丙烯酰胺凝胶电泳（2-DPAGE）和计算机辅助图像分析技术，研究了不同蚕色的雄蚕和雌蚕中部丝腺蛋白质表达的定性和定量差异。在两性中分辨出约1000个蛋白质点，并且显示大多数蛋白质分布在15 kDa至70 kDa和pH 4-8的区域。通过二维软件进行比较分析，发现部分蛋白质在黄茧中表达量较高，而2种蛋白质仅在雌蚕丝腺组织中表达。这些蛋白质主要存在于黄茧的雌蚕中部丝腺组织中。这些蛋白质可能参与了茧色表型的表达
Qualitative and quantitative differences in proteins expressed in the middle silkglands of male and female silkworm larvae that differ in silk colour were investigated by high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), followed by computer assisted image analysis. About 1000 protein spots were resolved in both the sexes and most proteins were shown to be distributed in the area from 15 kDa to 70 kDa and pH 4–8. It was found that some proteins displayed higher expression in yellow cocoon, while two proteins were only expressed in female silkworm silkgland tissue through the comparison and analysis by two-D software. These proteins especially existed in female silkworm middle silkgland tissue of yellow cocoon. Furthermore, these proteins might be involved in the expression of cocoon colour phenotype