Expression and localization of aquaporin-5 in the epithelial ovarian tumors
Expression and localization of aquaporin-5 in the epithelial ovarian tumors
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DOI：
10.1016/j.ygyno.2005.08.054
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发表时间：
2006-02-01
影响因子：
4.7
通讯作者：
Deng, L
中科院分区：
文献类型：
--
作者：
Yang, JH;Shi, YF;Deng, L
Objective.探讨水通道蛋白5（AQP 5）在卵巢上皮性肿瘤中的表达、定位及其临床意义。采用免疫组化、Western blotting和RT-PCR方法分别检测65例卵巢上皮性肿瘤和13例正常卵巢组织中AQP 5蛋白和mRNA的表达。AQP 5主要定位于良性肿瘤细胞的基底膜、交界性细胞的顶膜和基底膜，散在分布于恶性肿瘤细胞的膜上，而在正常卵巢上皮几乎不表达或表达较弱。AQP 5在卵巢恶性肿瘤和交界性肿瘤中的表达明显高于良性肿瘤（P < 0.05）和正常组织（P < 0.05）。在卵巢上皮性恶性肿瘤中，腹水量> 1000 ml者AQP 5表达高于腹水量< 500 ml者（P < 0.05）。AQP 5蛋白表达与淋巴结转移有关（P < 0.05）。AQP 5蛋白和mRNA的表达与腹水量呈正相关（P < 0.05），与淋巴结转移呈正相关（P < 0.05）。AQP 5的表达与FIGO分期、分级及组织学类型无关（P > 0.05）。提示AQP 5的过度表达在卵巢上皮性肿瘤的发生发展中起重要作用，可能与卵巢癌腹水形成有关。(c)2005年爱思唯尔公司All rights reserved.
Objective. To investigate the expression and localization of aquaporin-5 (AQP5) in epithelial ovarian tumors and its clinic significance.Methods. The expression of AQP5 protein and mRNA in 65 cases epithelial ovarian tumors and 13 cases normal tissue were measured by immunohistochemical technique, Western blotting and RT-PCR, respectively.Results. AQP5 is mainly localized in the basolateral membranes of benign tumor cells, the apical and basolateral membrane of borderline cells and scattered in the membrane of malignant cells and almost no or weak staining in normal ovarian epithelium. The AQP5 expression in ovarian malignant and borderline tumors was significantly higher than that of benign tumors (P < 0.05) and normal tissue (P < 0.05). Of all the epithelial ovarian malignant tumors, the AQP5 expression in cases with ascites volume more than 1000 ml was higher than that of ascites volume less than 500 ml (P < 0.05). Increased AQP5 protein level was associated with lymph node metastasis (P < 0.05). There is a positive correlation between ascites amount and the expression of AQP5 protein and mRNA (P < 0.05), as well as lymph node metastasis and the expression of AQP5 protein and mRNA (P < 0.05). The AQP5 expression was not related with FIGO stage, grade and histological type (P > 0.05).Conclusion. The data suggest that overexpression of AQP5 play an important role in tumorigenesis of epithelial ovarian tumors, which may be related to the ascites fort-nation of ovarian carcinoma. (c) 2005 Elsevier Inc. All rights reserved.