Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin Cry1Ac1
Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin Cry1Ac1
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DOI：
10.1046/j.1365-2583.2002.00373.x
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发表时间：
2002-12-01
影响因子：
2.6
通讯作者：
Ellar, D
中科院分区：
文献类型：
--
作者：
Gill, M;Ellar, D
苏云金芽孢杆菌合成的毒素（δ-内毒素）对昆虫具有高度特异性。一旦被摄入，毒素的活化形式与位于中肠上皮细胞上的特异性受体结合，插入膜中，导致形成渗漏孔并最终导致易感昆虫幼虫死亡。烟草天蛾幼虫对Cry 1Ac 1高度敏感，Cry 1Ac 1是一种被认为能结合烟草天蛾的毒素。sexta氨肽酶N，一种位于顶膜上的糖蛋白。然而，迄今为止获得的结合数据仅支持Cry 1Ac 1与APN在体外的相互作用。为了探索APN在体内的作用，我们利用GAL 4增强子捕获技术来驱动M. Sexta APN在Cry 1Ac 1不敏感果蝇幼虫的中肠和中胚层组织中的表达。喂食毒素的转基因果蝇现在被杀死，证明APN可以在体内作为Cry 1Ac 1的受体发挥作用。
The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of the susceptible insect larvae. Manduca sexta larvae are highly susceptible to Cry1Ac1, a toxin that is believed to bind M. sexta Aminopeptidase N, a glycoprotein located on the apical membrane. However, the binding data obtained to date only support the interaction of Cry1Ac1 with APN in vitro. To explore the in vivo role of APN, we have utilized the GAL4 enhancer trap technique to drive the expression of M. sexta APN in both midgut and mesodermal tissues of Cry1Ac1 insensitive Drosophila larvae. Transgenic Drosophila fed the toxin were now killed, demonstrating that APN can function as a receptor for Cry1Ac1 in vivo.