Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.
Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.
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Rab14 作为癌基因并通过 AKT 信号通路诱导胃癌细胞增殖
DOI：
10.1371/journal.pone.0170620
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Huang C
中科院分区：
文献类型：
--
作者：
Guo B;Wang W;Zhao Z;Li Q;Zhou K;Zhao L;Wang L;Yang J;Huang C
Rab 14是RAS癌基因家族成员之一，其功能异常与多种肿瘤的发生有关。但其在胃癌中的表达及功能尚不清楚。本研究旨在探讨Rab 14在胃癌细胞系中的作用及其机制。采用实时荧光定量PCR（qRT-PCR）检测17例胃腺癌组织和4株胃腺癌细胞系中Rab 14的表达。采用MTT法、集落形成实验和流式细胞术检测3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）对BGC-823和SGC-7901细胞增殖、细胞周期转换和凋亡的影响。Western blot检测Rab 14的调控途径和机制。在这项研究中，我们发现Rab 14在胃癌配对组织样本和细胞系中表达显著上调。当我们在SGC-7901细胞中过表达Rab 14或在BGC-823细胞中沉默Rab 14时，我们发现Rab 14可以改变细胞的生长、细胞周期或凋亡，并对AKT信号通路中的CCND 1、CDK 2和BAX有明显的调节作用。本研究为Rab 14作为一种新的肿瘤癌基因在胃癌中发挥作用提供了新的证据，并可能成为胃癌治疗的潜在靶点。
Rab14 is a member of RAS oncogene family, and its dysfunction has been reported to be involved in various types of human cancer. However, its expression and function were still unclear in gastric cancer. The aim of this study was to investigate the function and mechanism of Rab14 in gastric cancer cell lines. Quantitative real-time PCR (qRT-PCR) was performed in 17 gastric adenocarcinoma tissues and 4 cell lines to detect the expression of Rab14. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT), colony formation and flow cytometry assays were employed to determine the proliferative ability, cell cycle transition and apoptosis in vitro in BGC-823 or SGC-7901 cells. Western blot was performed to investigate the pathways and mechanism of Rab14 regulation. In this study, we show that Rab14 presents a significant up-regulated expression among the paired tissue samples and cell lines in gastric cancer. When we overexpressed Rab14 in SGC-7901 cells or silenced Rab14 in BGC-823 cells, we found that Rab14 could modify cell growth, cell cycle or apoptosis, which accompanied with an obvious regulation of CCND1, CDK2 and BAX involving in AKT signaling pathway. In conclusion, this study provides a new evidence on that Rab14 functions as a novel tumor oncogene and could be a potential therapeutic target in gastric cancer.