Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli

Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli
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DOI:
10.1128/jb.180.4.985-988.1998
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发表时间:
1998-02-01
影响因子:
3.2
通讯作者:
Eisenbach, M
Eisenbach, M
中科院分区:
生物学3区
文献类型:
--
作者:
Barak, R;Abouhamad, WN;Eisenbach, M

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大肠杆菌菌株过量生产的响应调节剂CheY响应乙酸通过增加他们的顺时针方向的鞭毛旋转偏置,即使当他们缺乏其他趋化蛋白,与乙酸代谢突变体,我们表明,乙酸激酶和乙酰辅酶A合成酶都参与了这一反应,因此,观察到的响应时,这些酶之一是失踪,但不是当两者都缺席。
Escherichia coli strains overproducing the response regulator CheY respond to acetate by increasing their clockwise bias of flagellar rotation, even when they lack other chemotaxis proteins, With acetate metabolism mutants, we demonstrate that both acetate kinase and acetyl coenzyme A synthetase are involved in this response, Thus, a response was observed when one of these enzymes was missing but not when both were absent.