In vitro studies of the action of antibiotics on Rickettsia prowazeki by two basic methods of cell culture.
In vitro studies of the action of antibiotics on Rickettsia prowazeki by two basic methods of cell culture.
复制标题
通过两种基本细胞培养方法进行抗生素对普瓦泽立克次体作用的体外研究。
DOI：
10.1093/infdis/130.6.564
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
W. Walsh
中科院分区：
文献类型：
--
作者：
C. Wisseman;A. Waddell;W. Walsh
建立了培养鸡胚细胞的小室法，并对立克次体菌斑技术进行了改进。这些方法允许检测抗生素诱导的形态学变化，并确定最小抑菌浓度和立克次体的杀灭率。对于普氏立克次体，氯霉素、强力霉素、红霉素和利福平的最小抑制浓度分别为1.0、0.1、0.06和0.008 μ g/ml。多西环素和米诺环素的体外作用与四环素的体外作用没有区别。多西环素、利福平和红霉素在临床可达到的浓度下可杀死立克次体，但在通常与治疗斑疹伤寒相关的条件下，其作用速度太慢，无法从人类宿主中根除该生物体。
chamber method of culturing chick embryo cells was developed, and the technique used to plaque Rickettsia was modified. These methods permitted the detection of morphologic changes induced by antibiotics and the determination of minimal inhibitory concentrations and rates of killing of rickettsiae. With Rickettsia prowazeki the minimal inhibitory concentrations were 1.0, 0.1, 0.06, and 0.008 [tg/ml for chloramphenicol, doxycycline, erythromycin, and rifampin, respectively. The in vitro actions of doxycycline and minocycline were indistinguishable from that of tetracycline. Doxycycline, rifampin, and erythromycin killed rickettsiae at clinically attainable concentrations, but the rates of action were too slow for eradication of the organisms from the human host under conditions usually associated with the treatment of typhus fever.