Suppression of drug-resistant staphylococcal infections by the quorum-sensing inhibitor RNAIII-inhibiting peptide
Suppression of drug-resistant staphylococcal infections by the quorum-sensing inhibitor RNAIII-inhibiting peptide
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DOI：
10.1086/386546
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发表时间：
2004-07-15
影响因子：
6.4
通讯作者：
Balaban, N
中科院分区：
文献类型：
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作者：
Dell'Acqua, G;Giacometti, A;Balaban, N
金黄色葡萄球菌和表皮葡萄球菌是与留置医疗器械上形成的生物膜相关的感染的主要原因。此类感染是发病和死亡的常见原因，并且由于生物膜对抗生素产生耐药性，因此难以治疗。 RNAIII 抑制肽 (RIP) (YSPWTNF-NH2) 通过破坏细菌细胞间通讯（称为“群体感应”）来抑制葡萄球菌的发病机制。使用血管移植大鼠模型，我们表明局部和全身应用 RIP 可以完全抑制耐药金黄色葡萄球菌和表皮葡萄球菌生物膜。本研究首次直接证明，使用群体感应抑制剂干扰细胞间通讯可以消除医疗设备相关的葡萄球菌感染。我们建议医疗器械可以涂有 RIP 来预防感染，包括耐抗生素葡萄球菌菌株引起的感染。
Staphylococcus aureus and S. epidermidis are major causes of infection related to biofilm formed on indwelling medical devices. Such infections are common causes of morbidity and mortality and, because of biofilm resistance to antibiotics, are difficult to treat. The RNAIII-inhibiting peptide (RIP) (YSPWTNF-NH2) inhibits the pathogenesis of staphylococci by disrupting bacterial cell-cell communication ( known as "quorum sensing"). Using a vascular-graft rat model, we show that RIP, applied locally and systemically, can completely inhibit drug-resistant S. aureus and S. epidermidis biofilms. The present study provides the first direct demonstration that interfering with cell-cell communication by use of a quorum-sensing inhibitor can eliminate medical device - associated staphylococcal infections. We suggest that medical devices could be coated with RIP to prevent infections, including those by antibiotic-resistant staphylococcal strains.