Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci
Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci
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DOI：
10.1094/phyto.2001.91.2.188
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发表时间：
2001-02-01
期刊：
影响因子：
3.2
通讯作者：
Czosnek, H
中科院分区：
文献类型：
--
作者：
Ghanim, M;Morin, S;Czosnek, H
B型烟粉虱与受感染的番茄植株同笼饲养8 h后，可传播番茄黄卷叶病毒（TYLCV）。在这一潜伏期，TYLCV在被认为与烟粉虱病毒易位有关的器官中传播。在延长侵染番茄植株的获取期（AAPs）后，对昆虫的柱头、头部、中肠、血淋巴和唾液腺进行不加任何处理的聚合酶链反应（PCR）；用病毒特异性引物评估TYLCV的存在。在AAP 10min后，首次在烟粉虱头部检测到TYLCV DNA。40分钟后病毒出现在中肠，90分钟后在血淋巴中首次检测到病毒。首次在血淋巴中检测到TYLCV， 5.5 h后在唾液腺中发现。对昆虫器官进行免疫捕获- pcr，发现病毒衣壳蛋白与病毒基因组同时存在于昆虫器官中，这表明至少有部分TYLCV作为病毒粒子易位。虽然雌性作为载体比雄性更有效，但在大致相同的AAP后，在雄性和雌性的唾液腺中检测到TYLCV。
Whiteflies (Bemisia tabaci, biotype B) were able to transmit Tomato yellow leaf curl virus (TYLCV) 8 h after they were caged with infected tomato plants. The spread of TYLCV during this latent period was followed in organs thought to be involved in the translocation of the virus in B. tabaci. After increasing acquisition access periods (AAPs) on infected tomato plants, the stylets, the head, the midgut, a hemolymph sample, and the salivary glands dissected from individual insects were subjected to polymerase chain reaction (PCR) without any treatment; the presence of TYLCV was assessed with virus-specific primers. TYLCV DNA was first detected in the head of B. tabaci after a 10-min AAP. The virus was present in the midgut after 40 min and was first detected in the hemolymph after 90 min. TYLCV was found in the salivary glands 5.5 h after it was first detected in the hemolymph. Subjecting the insect organs to immunocapture-PCR showed that the virus capsid protein was in the insect organs at the same time as the virus genome, suggesting that at least some TYLCV translocates as virions. Although females are more efficient as vectors than males, TYLCV was detected in the salivary glands of males and of females after approximately the same AAP.