A P1-BASED PHYSICAL MAP OF THE REGION FROM D17S776 TO D17S78 CONTAINING THE BREAST-CANCER SUSCEPTIBILITY GENE BRCA1
A P1-BASED PHYSICAL MAP OF THE REGION FROM D17S776 TO D17S78 CONTAINING THE BREAST-CANCER SUSCEPTIBILITY GENE BRCA1
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DOI：
10.1093/hmg/3.11.1919
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发表时间：
1994-11-01
影响因子：
3.5
通讯作者：
GOLDGAR, DE
中科院分区：
文献类型：
--
作者：
NEUHAUSEN, SL;SWENSEN, J;GOLDGAR, DE
BRCA1是一种乳腺癌和卵巢癌易感位点，已被分离出来，定位于17q21。构建了BRCA1区域的物理图谱，从近端边界D17S776延伸到远端边界D17S78，包括P1和YAC端51个序列标记位点（STSs）、9个新的短串联重复（STR）多态性标记和8个鉴定基因。该群落横跨约2.3 Mb的区域，包含29个p1、11个yac、2个bac和1个cosmid。基于两个连锁家族的关键重组，BRCA1进一步定位于近端D17S1321和远端D17S1325交界的区域。在这个估计的600kb区域内，该序列完全由P1s和bac组成，通过sts内容图谱排序，并通过DNA限制性片段指纹图谱证实。
BRCA1, a breast and ovarian cancer susceptibility locus, has been isolated and maps to 17q21. A physical map of the BRCA1 region which extended from the proximal boundary at D17S776 to the distal boundary at D17S78 was constructed and consists of 51 sequence tagged sites (STSs) from P1 and YAC ends, nine new short-tandem repeat (STR) polymorphic markers, and eight identified genes. The contig, which spans the estimated 2.3 Mb region, contains 29 P1s, 11 YACs, two BACs, and one cosmid. Based on key recombinants in two linked families, BRCA1 was further localized to a region bounded by D17S1321 on the proximal side and D17S1325 on the distal side. Within this estimated 600 kb region, the contig was composed completely of P1s and BACs ordered by STS-content mapping and confirmed by DNA restriction fragment fingerprinting.