Nociceptive spinal neurons expressing NKI and GABAB receptors are located in lamina I
Nociceptive spinal neurons expressing NKI and GABAB receptors are located in lamina I
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DOI：
10.1016/j.brainres.2003.12.027
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发表时间：
2004-04-02
期刊：
影响因子：
2.9
通讯作者：
Tavares, I
中科院分区：
文献类型：
--
作者：
Castro, AR;Pinto, M;Tavares, I
在大鼠中评价了表达NK 1和γ-氨基丁酸（GABA）（B）受体的脊髓背角神经元的伤害性。Fos蛋白的免疫检测，由皮肤的有害机械刺激诱导，结合NK 1或GABA（B）受体（双重免疫染色研究）或两种受体（三重免疫染色研究）的免疫细胞化学。Fos和每种受体双标记的神经元主要分布在I层。NK 1或GABA（B）受体免疫染色Fos阳性细胞的比例在椎板I高于其余脊髓椎板。在背角板层中，GABA（B）受体的Fos阳性细胞多于NK 1。在三重免疫染色研究中，NK 1和GABA（B）受体的共定位仅发生在板层I中，并且在表达Fos的神经元中更高。在形态学上，I层细胞中，NK 1阳性细胞主要为梭形。而梭形、锥体和扁平型NK 1神经元表达GABA（B）受体的比例相近。在伤害性激活的程度方面，这些细胞类型之间没有发现差异。结果提示，NK 1和GABA（B）受体共定位是第一层梭形、锥体和扁平神经元的共同特征。考虑到这三类细胞在不同上行系统中的参与，我们认为P物质（SP）和GABA的同时作用可能在I层脊髓上传递的伤害性信息的调制中起重要作用。(C)2004 Elsevier B. V.保留所有权利。
The nociceptive nature of spinal dorsal horn neurons expressing NK1 and gamma-aminobutyric acid (GABA)(B) receptors was evaluated in the rat. Immunodetection of the Fos protein, induced by noxious mechanical stimulation of the skin, was combined with immunocytochemistry for NK1 or GABA(B) receptors (double-immunostaining study) or both receptors (triple-immunostaining study). Neurons double-labeled for Fos and for each receptor largely prevailed in lamina I. The proportions of Fos-positive cells immunostained for NK1 or GABA(B) receptors were higher in lamina I than in the remaining spinal laminae. More Fos-positive cells were immunoreactive (IR) for GABA(B) receptors than for NK1 in all dorsal horn laminae. In the triple-immunostaing study, co-localization of NK1 and GABA(B) receptors occurred only in lamina I and was higher in neurons expressing Fos. As to the morphological lamina I cell class, NK1-positive cells belonged mainly to the fusiform. type while similar proportions of fusiform, pyramidal and flattened NK1 neurons expressed GABA(B) receptors. No differences were found between those cell types as to the degree of nociceptive activation. The present results suggest that the co-localization of NK1 and GABA(B) receptors is a common feature of fusiform, pyramidal and flattened neurons in lamina I. Considering the participation of the three cell classes in various ascending systems, it is concluded that a simultaneous action of substance P (SP) and GABA may play an important role in the modulation of nociceptive input supraspinally transmitted from lamina I. (C) 2004 Elsevier B.V. All rights reserved.