Synthesis of tunable and multifunctional Ni-doped near-infrared QDs for cancer cell targeting and cellular sorting.
Synthesis of tunable and multifunctional Ni-doped near-infrared QDs for cancer cell targeting and cellular sorting.
复制标题
DOI：
10.1021/bc200435e
复制
发表时间：
2012-02
影响因子：
4.7
通讯作者：
Narendra Pratap Singh;S. Charan;K. Sanjiv;Shih-Hsin Huang;Yu-Chu Hsiao;C. Kuo;F. Chien;Terrence C Lee-Terr
中科院分区：
文献类型：
--
作者：
Narendra Pratap Singh;S. Charan;K. Sanjiv;Shih-Hsin Huang;Yu-Chu Hsiao;C. Kuo;F. Chien;Terrence C Lee-Terr
在这里，我们报告了可调磁性Ni掺杂近红外（NIR）量子点（MNIR-QD）作为一种有效的探针，用于靶向，成像和细胞分选应用的简便制备。我们通过热胶体合成方法合成了MNIR-QDs，得到单分散和可调的QDs。对这些疏水性量子点进行了结构和组成表征，并进一步用氨基-PEG和羧基-PEG官能化，以改善其生物相容性。由于量子点是已知的有毒的，由于镉的存在下，我们已经评估了在体外和体内的毒性，我们的表面官能化的MNIR-QD。我们的研究结果表明，表面官能化的MNIR-QD在实验中使用的浓度下没有表现出显着的毒性，因此适合于生物应用。对于进一步的体外应用，我们通过NHS化学将叶酸共价连接到氨基-PEG-包被的MNIR-QD的表面，以靶向主要存在于HeLa细胞中的叶酸受体，以证明这些MNIR-QD的特异性靶向和磁性行为。与不含叶酸的相比，用叶酸连接的氨基PEG包被的MNIR QD（FA-PEG-MNIR-QD）处理HeLa细胞观察到特异性改善。由于合成的探针具有磁性，我们还成功地证明了使用磁体在吸收探针的细胞之间进行分选。我们的研究结果有力地表明，这些功能化的MNIR-QD可以是一个潜在的探针靶向，细胞分选和生物成像应用。
Here, we report the facile preparation of tunable magnetic Ni-doped near-infrared (NIR) quantum dots (MNIR-QDs) as an efficient probe for targeting, imaging, and cellular sorting applications. We synthesized the MNIR-QDs via a hot colloidal synthesis approach to yield monodisperse and tunable QDs. These hydrophobic QDs were structurally and compositionally characterized and further functionalized with amino-PEG and carboxyl-PEG to improve their biocompatibility. Since QDs are known to be toxic due to the presence of cadmium, we have evaluated the in vitro and in vivo toxicity of our surface-functionalized MNIR-QDs. Our results revealed that surface-functionalized MNIR-QDs did not exhibit significant toxicity at the concentrations used in the experiments and are therefore suitable for biological applications. For further in vitro applications, we covalently linked folic acid to the surface of amino-PEG-coated MNIR-QDs through NHS chemistry to target the folate receptors largely present in the HeLa cells to demonstrate the specific targeting and magnetic behavior of these MNIR-QDs. Improved specificity has been observed with treatment of HeLa cells with the folic acid-linked amino PEG-coated MNIR QDs (FA-PEG-MNIR-QDs) compared to the one without folic acid. Since the synthesized probe has magnetic property, we have also successfully demonstrated sorting between the cells which have taken up the probe with the use of a magnet. Our findings strongly suggest that these functionalized MNIR-QDs can be a potential probe for targeting, cellular sorting, and bioimaging applications.