Clinicopathological significance of combined analysis of cytokeratin19 expression and preoperative serum CYFRA21-1 levels in human lung squamous cell carcinoma.
Clinicopathological significance of combined analysis of cytokeratin19 expression and preoperative serum CYFRA21-1 levels in human lung squamous cell carcinoma.
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发表时间：
2013-06
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通讯作者：
Shoji Hanada;N. Nishiyama;S. Mizuguchi;S. Yamano;A. Kakehashi;M. Wei;Hidetoshi Inoue;Hiroaki Komatsu;Kyukwang Chung;S. Suehiro;H. Wanibuchi
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作者：
Shoji Hanada;N. Nishiyama;S. Mizuguchi;S. Yamano;A. Kakehashi;M. Wei;Hidetoshi Inoue;Hiroaki Komatsu;Kyukwang Chung;S. Suehiro;H. Wanibuchi
背景为了鉴定人肺鳞状细胞癌（SCC）的有用生物标志物，研究了细胞角蛋白19（CK 19）在人SCC组织中的表达。此外，我们还通过免疫组化检测了CK 19表达水平和术前血清CYFRA 21 -1水平的意义，以及它们与人肺鳞癌临床病理特征的相关性。方法采用QSTAR Elite LC-MS/MS技术对SCC癌组织和非癌组织中的蛋白质进行鉴定，为SCC的诊断和预后判断提供依据。CK 19的免疫染色被分类为“CK 19-强”或“CK 19-弱”。分析107例肺鳞癌患者肿瘤组织中CK 19表达及血清CYFRA 21-1浓度与预后的关系。结果：LCMS/MS法检测到人鳞状细胞癌组织中CK 19表达上调，免疫组化显示CK 19弱表达是肿瘤特异性生存率下降的重要预测因子（p = 0.032）。与其他组相比，肿瘤组织中CK 19免疫染色弱且血清CYFRA 21 -1浓度高的患者的预后明显较差（p = 0.003）。结论CK 19低表达和血清CYFRA 21 -1高水平的组合是人肺SCC患者预后不良的预测因子。
BACKGROUND To identify a useful biomarker for human lung squamous cell carcinoma (SCC), the expression of cytokeratin19 (CK19) in human SCC tissue was investigated. In addition, we examined the significance of CK19 expression levels by immunostaining and CYFRA21-1 levels in preoperative serum, and their correlation with the clinicopathologic features of human lung SCC. METHODS To identify proteins in cancerous and non-cancerous tissues for the diagnosis and prognosis of SCC, QSTAR Elite LC-MS/MS was used. Immunostaining for CK19 was classified as either "CK19-strong" or "CK19-weak". Correlations between prognosis and both CK19 expression in tumor tissues and serum concentrations of CYFRA 21-1 were analyzed in 107 cases of lung SCC. RESULTS The upregulation of CK19 in human squamous cell carcinoma tissues was observed by LCMS/MS. The weak expression of CK19, as determined by immunostaining intensity, was a significant predictor of poorer disease-specific survival (p = 0.032). The prognosis was significantly poorer for patients with weak CK19 immunostaining in tumor tissues and a high serum concentration of CYFRA21-1 compared with the other groups (p = 0.003). CONCLUSIONS The combination of weak CK19 expression and high serum CYFRA21-1 levels is a predictor of poorer prognosis for patients with human lung SCC.