LC-MS-MS Analysis of Strictosamide in Rat Plasma, and Application of the Method to a Pharmacokinetic Study
LC-MS-MS Analysis of Strictosamide in Rat Plasma, and Application of the Method to a Pharmacokinetic Study
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DOI：
10.1365/s10337-009-0980-8
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发表时间：
2009-05-01
期刊：
影响因子：
1.7
通讯作者：
Bi, Kai-Shun
中科院分区：
文献类型：
--
作者：
Hu, Xin;Lv, Yun-Feng;Bi, Kai-Shun
建立了一种快速、简便的高效液相色谱-串联质谱法测定大鼠血浆中异柳胺浓度。在C-18柱上，用甲醇、水和含0.05%乙酸的乙腈的混合物进行梯度洗脱，实现色谱分离。地高辛用作内标。选择反应监测（SRM）用于MS定量。在大鼠血浆中，在0.05-20 ng mL（-1）范围内线性良好。定量下限为0.04 ng mL（-1）。该方法精密度高、可靠性好，可用于药动学研究。
A rapid and simple high-performance liquid chromatographic tandem mass spectrometric method has been developed and validated for analysis of strictosamide in rat plasma. Chromatographic separation was achieved on a C-18 column by gradient elution with mixtures of methanol, water, and acetonitrile containing 0.05% acetic acid. Digoxin was used as internal standard. Selected reaction monitoring (SRM) was used for MS quantitation. Linearity was good in the range 0.05-20 ng mL(-1) in rat plasma. The lower limit of quantitation was 0.04 ng mL(-1). The method is precise and reliable and can be applied to pharmacokinetic studies.