Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16.
Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16.
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DOI：
10.3892/ijo.19.1.97
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发表时间：
2001-07
影响因子：
5.2
通讯作者：
H. Sashiyama;Y. Shino;Y. Kawamata;Y. Tomita;N. Ogawa;H. Shimada;S. Kobayashi;T. Asano;T. Ochiai;H. Shirasawa
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文献类型：
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作者：
H. Sashiyama;Y. Shino;Y. Kawamata;Y. Tomita;N. Ogawa;H. Shimada;S. Kobayashi;T. Asano;T. Ochiai;H. Shirasawa
用重组腺病毒将人乳头瘤病毒16型（HPV 16）E6/E7导入原代培养的人食管角朊细胞，可延长细胞的寿命，而未处理的细胞在14-16个群体倍增时间（PDL）内衰老。在50至100个PDL之间寿命延长的食管角质形成细胞中观察到端粒酶活性上调和获得抗血清生长，并且在100个PDL后急剧增加。与p53密码子72处的Pro/Pro多态性的角质形成细胞样品显示出对HPV 16 E6/E7诱导的寿命延长和永生化的抗性，而与p53 Arg/Arg或Arg/Pro多态性的其他角质形成细胞样品则没有。腺病毒载体诱导E6/E7的高效率也揭示了角质形成细胞永生化的M1和M2阶段。
Transduction of human papillomavirus type 16 (HPV16) E6/E7 into primary culture of human esophageal keratinocytes using a recombinant adenovirus prolonged the life-span, while untreated cells senesced within 14-16 population doublings (PDLs). Up-regulation of telomerase activity and acquisition of serum-resistant growth were observed in the esophageal keratinocytes with extended life-span between 50 and 100 PDLs, and drastically increased after 100 PDLs. A keratinocyte sample with a polymorphism of Pro/Pro at codon 72 of p53 showed resistance to HPV16 E6/E7-induced life-span-extension and immortalization, in contrast to others with p53 polymorphisms of Arg/Arg or Arg/Pro, which did not. The high efficiency of E6/E7-induction by adenovirus vector also revealed the M1 and M2 stages of keratinocyte immortalization first described in this report.