Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti.
Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti.
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DOI：
10.1093/jhered/esr134
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
D. Shin;A. Mori;D. Severson
中科院分区：
文献类型：
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作者：
D. Shin;A. Mori;D. Severson
登革热和黄热病媒介蚊子埃及伊蚊中的内源性减数分裂驱动因子可以在来自合适遗传背景的杂交中引起高度男性偏向的性比扭曲。我们先前选择了一个菌株，该菌株携带与A中1号染色体上的雄性决定等位基因（M）连锁的强减数分裂驱动基因（D）。埃及人。在此，我们对来自驾驶员雄性和驾驶敏感雌性的回交（BC（1））后代中的M（D）基因座进行了分离分析。BC（2）后代的性比分析表明，M（D）基因座与性别决定基因座之间的重组率为5.2%。该区域的多点连锁图谱显示了与现有参考连锁图谱一致的标记顺序和重组频率，并将M（D）位点置于由LF159位点和微卫星标记446GAA定义的6.5 cm间隔内，这将有助于未来的定位克隆工作。
An endogenous meiotic driver in the dengue and yellow fever vector mosquito Aedes aegypti can cause highly male-biased sex ratio distortion in crosses from suitable genetic backgrounds. We previously selected a strain that carries a strong meiotic drive gene (D) linked with the male-determining allele (M) on chromosome 1 in A. aegypti. Here, we performed segregation analysis of the M(D) locus among backcross (BC(1)) progeny from a driver male and drive-sensitive females. Assessment of sex ratios among BC(2) progeny showed ∼5.2% recombination between the M(D) locus and the sex determination locus. Multipoint linkage mapping across this region revealed consistent marker orders and recombination frequencies with the existing reference linkage map and placed the M(D) locus within a 6.5-cm interval defined by the LF159 locus and microsatellite marker 446GAA, which should facilitate future positional cloning efforts.