Intracapsular Organization of Ciliary Zonules in Monkey Eyes
Intracapsular Organization of Ciliary Zonules in Monkey Eyes
复制标题
DOI：
10.1002/ar.21220
复制
发表时间：
2010-10-01
影响因子：
2
通讯作者：
Takada, Masahiko
中科院分区：
文献类型：
--
作者：
Hiraoka, Mari;Inoue, Ken-Ichi;Takada, Masahiko
睫状带负责改变眼睛屈光焦点处晶状体的曲率。目前已建立的理论是基于附着在囊膜表面的索状浅纤维束的松弛，从而引起晶状体前后曲率的变化，导致晶状体材料的自发液体运动。为了在任何距离实现精确对焦，应该存在一个更活跃的职能组织。本研究不仅测定了猴眼上透明带的表面附着，而且还测定了透明带在囊内的附着。此外，还分析了聚焦在新生儿和老花眼中的发展。采用常规免疫荧光染色、分子免疫荧光染色、冰冻切片原位杂交等方法对含纤维蛋白-1(FBN-1)的透明带(FBN-1)和含IV型胶原(Col IV)的晶状体囊(Col IV)进行组织学观察。睫状突与晶状体前/后囊膜之间呈放射状环状附着物，呈三角形。在它们的上方发现了两个功能性的层内整合：横跨赤道的前后交叉纤维和分布于前极区或后极区的放射状纤维。这些纤维与晶状体上皮附近的深层结缔组织紧密结合。细小的带状纤维聚集在一起，逐渐形成束状，并在通往胶囊的途中再次分叉。束中的横向条纹染色图案表明它们的弹性性质。晶状体前囊膜和晶状体后囊膜的IV型胶原α螺旋免疫反应在α-1、2、4比α-3、5、6更强。新生眼晶状体表面可见细小的膜性小带和纤毛突起，而不是束状。ISH分析显示，在睫状突无色素上皮细胞胞浆中有FBN 1mRNA的高合成表达。FBN-1的合成表达随增龄而下降。根据调节机制，前囊或后囊的主动动态运动主要通过两个层内的带状整合改变晶状体的形态，包括赤道上的前后交叉纤维和向前或后极区的放射状纤维与环状或纵向睫状肌的协调收缩。聚焦的发育变化是由新生儿眼中FBN-1的合成引起的。Anat Rec，293：1797-1804,2010。(C)2010年Wiley-Liss公司
Ciliary zonules are responsible for changing the curvature of a lens in the dioptric focus of an eye. Present established theory is based on the relaxation of zonular superficial fasciculi affixed to the capsular surface, thereby inducing the change of anterior- and posterior lens curvature causing spontaneous liquid movement of lens material. To achieve precise focusing at any distance, a more active functional organization should exist. The present studies were performed to determine not only the surface attachment but also the intracapsular affix of zonules on monkey eyes. In addition, the development of focusing in newborn and presbyopia is analyzed. Histology was prepared by conventional and molecular immunofluorescence stainings on the compositions of zonules with fibrillin-1 (FBN 1) and lens capsule with collagen IV (COL IV), and in situ hybridization (ISH) analyses on frozen sections. Superficial circumferential attachments of zonule were found radially oriented between ciliary processes and anterior/posterior lens capsules forming a triangular figure. Two functional intralayer integrations were found above them; anterior-posterior crossed fibers over the equator and radial fibers distributed toward the anterior or posterior polar areas. These fibers were bound tightly to the deep layer connective tissues close to the lens epithelium. Fine zonular fibers were aggregated, gradually forming bundles and bifurcated again on the way to the capsule. The lateral striped staining pattern in bundles suggested their elastic nature. Response of alpha-helixes of collagen IV immunostaining was more positive on alpha-1,2,4 than alpha-3,5,6 on anterior- and posterior lens capsules. Newborn eyes revealed not fascicular but fine membranous zonules on the lens surface and small ciliary processes. ISH analysis revealed high synthetic expression of FBN 1 mRNA in cytoplasm of nonpigmented epithelial cells of ciliary processes. The synthetic expression of FBN 1 declined with aging. According to the mechanism of accommodation, active dynamic movement of anterior or posterior capsules play the main role of changing the lens configuration by two intralayer zonular integrations, including anterior-posterior crossed fibers over the equator and radial fibers toward anterior or posterior polar areas acting with coordinated contraction of circular or longitudinal ciliary muscles. The developmental change on focusing is brought about by synthesis of FBN 1 in the newborn eye. Anat Rec, 293:1797-1804, 2010. (C) 2010 Wiley-Liss, Inc.