The investigation of the interactions between CdSe quantum dots and human serum albumin by resonance Rayleigh scattering and second-order scattering spectra.
The investigation of the interactions between CdSe quantum dots and human serum albumin by resonance Rayleigh scattering and second-order scattering spectra.
复制标题
DOI：
10.1016/j.saa.2009.12.047
复制
发表时间：
2010-03
期刊：
影响因子：
--
通讯作者：
Ke-Jing Huang;Cai-Yun Wei;Yantao Shi;W. Xie;Wei Wang
中科院分区：
文献类型：
--
作者：
Ke-Jing Huang;Cai-Yun Wei;Yantao Shi;W. Xie;Wei Wang
人血清白蛋白(HSA)是人体血浆中含量最丰富的蛋白质，具有重要的生理功能。在Tris-HCl缓冲溶液(pH 7.4)中，水溶性半导体CdSe量子点(Qds)与人血清白蛋白(HSA)反应，导致共振瑞利散射(RRS)和二阶散射(SOS)强度显著增强。在此基础上，发展了一种新的研究量子点与人血清白蛋白相互作用的方法。优化了测定参数，并对反应机理进行了探讨。在最佳条件下，散射强度增量(ΔI)与人血清白蛋白浓度在0.4~4 8.0μ−1范围内呈良好的线性关系，检出限分别为0.1 0μ摩尔−1和0.2 5μ摩尔−1。该方法灵敏、简便、快速。该方法已成功应用于人尿样中人血清白蛋白的测定。用这种新的分析方法获得了满意的结果。
Human serum albumin (HSA) was the most abundant protein in human plasma and has significant physiological function. In Tris–HCl buffer solution (pH 7.4), water-soluble semiconductor CdSe quantum dots (QDs) reacted with HSA and the products resulted in a great enhancement of the intensity of resonance Rayleigh scattering (RRS) and second-order scattering (SOS). Based on this, a new method was developed to investigate the interactions between QDs and HSA. The parameters with regard to determination were optimized, and the reaction mechanism was discussed. Under optimal conditions, the increments of scattering intensity (ΔI) were directly proportional to the concentrations of HSA in the range of 0.4–48.0μmolL−1. The detection limits were 0.10μmolL−1for RRS method and 0.25μmolL−1for SOS method. The proposed method was sensitive, simple and rapid. It has been successfully applied to the determination of HSA in human urine samples. Analytical results obtained with this novel assay were satisfactory.