Correlating genomic copy number alterations with clinicopathologic findings in 75 cases of hepatocellular carcinoma.
Correlating genomic copy number alterations with clinicopathologic findings in 75 cases of hepatocellular carcinoma.
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75 例肝细胞癌基因组拷贝数变化与临床病理结果的关联
DOI：
10.1186/s12920-021-00998-9
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发表时间：
2021-06-08
影响因子：
2.7
通讯作者：
Hu Q
中科院分区：
文献类型：
--
作者：
Peng G;Chai H;Ji W;Lu Y;Wu S;Zhao H;Li P;Hu Q
背景寡核苷酸阵列比较基因组杂交（aCGH）技术已被用于检测各种肿瘤的体细胞拷贝数改变（CNA）。本研究旨在评估aCGH的临床实用性的情况下，肝细胞癌（HCC）和评估CNAs和临床病理findings.MethodsaCGH之间的相关性进行了75例肝癌与配对的DNA样本从肿瘤和邻近的非肿瘤组织。这些病例的生存结局基于BCLC、Edmondson-Steiner分级（E-S）和复发状态进行分析。CNAs与临床病理结果的相关性进行了分析Wilcoxon秩检验和聚类vs. Kmeans.ResultsThe生存结果表明，BCLC阶段和复发状态可以预测和E-S分级可以是HCC的修饰符。最常见的CNA涉及1 q和8 q的增加和16 q的损失（50%），4 q和17 p的损失和5 p的增加（40%），以及8 p和13 q的损失（30%）。对基因组图谱和聚类的分析表明，在A期、III级和无复发病例中观察到的4q13.2q35.2和10q22.3q26.13的丢失可能与良好的生存相关，而在C期病例中观察到的1p36.31p22.1的丢失和2q11.2q21.2和20p13p11.1的增加，结论aCGH分析可用于检测HCC患者复发的CNA，并涉及HCC患者的关键基因和通路。进一步分析大量病例系列以验证CNA与HCC临床病理结果的相关性，可以为解释CNA和预测预后提供信息。
BackgroundOligonucleotide array comparative genomic hybridization (aCGH) analysis has been used for detecting somatic copy number alterations (CNAs) in various types of tumors. This study aimed to assess the clinical utility of aCGH for cases of hepatocellular carcinoma (HCC) and to evaluate the correlation between CNAs and clinicopathologic findings.MethodsaCGH was performed on 75 HCC cases with paired DNA samples from tumor and adjacent nontumor tissues. Survival outcomes from these cases were analyzed based on Barcelona-Clinic Liver Cancer Stage (BCLC), Edmondson-Steiner grade (E-S), and recurrence status. Correlation of CNAs with clinicopathologic findings was analyzed by Wilcoxon rank test and clustering vs. K means.ResultsThe survival outcomes indicated that BCLC stages and recurrence status could be predictors and E-S grades could be a modifier for HCC. The most common CNAs involved gains of 1q and 8q and a loss of 16q (50%), losses of 4q and 17p and a gain of 5p (40%), and losses of 8p and 13q (30%). Analyses of genomic profiles and clusters identified that losses of 4q13.2q35.2 and 10q22.3q26.13 seen in cases of stage A, grade III and nonrecurrence were likely correlated with good survival, while loss of 1p36.31p22.1 and gains of 2q11.2q21.2 and 20p13p11.1 seen in cases of stage C, grade III and recurrence were possibly correlated with worst prognosis.ConclusionsThese results indicated that aCGH analysis could be used to detect recurrent CNAs and involved key genes and pathways in patients with HCC. Further analysis on a large case series to validate the correlation of CNAs with clinicopathologic findings of HCC could provide information to interpret CNAs and predict prognosis.
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影响因子：
12.6
作者：
Steinemann, Doris;Skawran, Britta;Wilkens, Ludwig
通讯作者：
Wilkens, Ludwig
影响因子：
3.7
作者：
Qi LN;Li LQ;Chen YY;Chen ZH;Bai T;Xiang BD;Qin X;Xiao KY;Peng MH;Liu ZM;Liu TW;Qin X;Li S;Han ZG;Mo ZN;Santella RM;Winkler CA;O'Brien SJ;Peng T
通讯作者：
Peng T
影响因子：
64.5
作者：
Cancer Genome Atlas Research Network. Electronic address: wheeler@bcm.edu;Cancer Genome Atlas Research Network
通讯作者：
Cancer Genome Atlas Research Network
影响因子：
1.3
作者：
Liu YJ;Zhou Y;Yeh MM
通讯作者：
Yeh MM
DOI：
10.1080/13651820410024058
发表时间：
2005-01-01
期刊：
HPB : the official journal of the International Hepato Pancreato Biliary Association
影响因子：
--
作者：
Pons, Fernando;Varela, Maria;Llovet, Josep M
通讯作者：
Llovet, Josep M