Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction
Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction
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DOI：
10.1186/2044-5040-1-20
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发表时间：
2011-01-01
期刊：
影响因子：
4.9
通讯作者：
Hoffman, Eric P.
中科院分区：
文献类型：
--
作者：
Mate, Suzanne E.;Brown, Kristy J.;Hoffman, Eric P.
背景资料：在发育过程中，加州电鳐的鳃中胚层转分化为一个能够产生高压放电的电器官。该器官含有高密度的胆碱能突触，并作为肌纤维膜特化的生物化学模型，神经肌肉接头（NMJ）。我们研究了基因组和蛋白质组的电器官，以了解其组成，以确定是否有一致性与骨骼肌和NMJ，并确定新的突触proteins.Results：的435个蛋白质鉴定，300映射到鱼雷cDNA序列与>= 2肽。我们确定了14个未知的蛋白质的电器官，是已知的乙酰胆碱受体聚集或信号转导中发挥作用。此外，两个人类开放阅读框架，C1orf 123和C6orf 130，显示出与电器官蛋白的高度序列相似性。我们的概况列出了几种在骨骼肌中高度表达或具有肌肉特异性的蛋白质。突触蛋白，如乙酰胆碱酯酶，乙酰胆碱受体亚基，和rapsyn存在于电器官蛋白质组，但在骨骼肌蛋白质组中缺席。结论：我们的综合基因组和蛋白质组学分析支持研究描述的肌肉样配置文件的器官。我们表明，它是一个存储库的NMJ蛋白质，但我们目前的限制，其使用作为一个全面的模型的NMJ。最后，我们确定了几种蛋白质，可能成为候选人的信号蛋白，以前没有表征为组件的NMJ。
Background: During development, the branchial mesoderm of Torpedo californica transdifferentiates into an electric organ capable of generating high voltage discharges to stun fish. The organ contains a high density of cholinergic synapses and has served as a biochemical model for the membrane specialization of myofibers, the neuromuscular junction (NMJ). We studied the genome and proteome of the electric organ to gain insight into its composition, to determine if there is concordance with skeletal muscle and the NMJ, and to identify novel synaptic proteins.Results: Of 435 proteins identified, 300 mapped to Torpedo cDNA sequences with >= 2 peptides. We identified 14 uncharacterized proteins in the electric organ that are known to play a role in acetylcholine receptor clustering or signal transduction. In addition, two human open reading frames, C1orf123 and C6orf130, showed high sequence similarity to electric organ proteins. Our profile lists several proteins that are highly expressed in skeletal muscle or are muscle specific. Synaptic proteins such as acetylcholinesterase, acetylcholine receptor subunits, and rapsyn were present in the electric organ proteome but absent in the skeletal muscle proteome.Conclusions: Our integrated genomic and proteomic analysis supports research describing a muscle-like profile of the organ. We show that it is a repository of NMJ proteins but we present limitations on its use as a comprehensive model of the NMJ. Finally, we identified several proteins that may become candidates for signaling proteins not previously characterized as components of the NMJ.