Effects of bromate on life history parameters, swimming speed and antioxidant biomarkers in Brachionus calyciflorus
Effects of bromate on life history parameters, swimming speed and antioxidant biomarkers in Brachionus calyciflorus
复制标题
溴酸盐对萼花臂尾轮生活史参数、游泳速度及抗氧化生物标志物的影响
DOI：
10.1016/j.ecoenv.2020.111705
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发表时间：
2021-01-15
影响因子：
6.8
通讯作者：
Wang, Xing-Ming
中科院分区：
文献类型：
--
作者：
Xu, Xiao-Ping;Chen, Tao;Wang, Xing-Ming
烘焙行业和自来水消毒会向地表水中释放相当数量的溴酸盐，据报道，溴酸盐是一种对哺乳动物具有致癌作用的化合物。轮虫在淡水生态系统中扮演着重要的角色，是评估环境污染的模式生物。研究了不同浓度(0.001、0.0 1、0.1、1、10、10 0、2 0 0 mg/L)的溴酸盐对萼花臂尾轮虫生命表和种群生长参数的影响。结果表明，溴酸盐对花苞假单胞菌的24 h半数致死量为365.29 mg/L(95%CI：290.37~480.24)。0.01、10和200 mg/L处理缩短了生殖周期。高浓度溴酸盐(10 0 mg/L和2 0 0 mg/L)显著降低了萼花臂尾轮虫的净繁殖率、内禀种群增长率、寿命和混交率。为了探讨其潜在的机制，我们比较了溴酸盐处理组和对照组的游泳速度和抗氧化生物标志物。结果表明，暴露于溴酸盐后，仔鱼体内的谷胱甘肽(GSH)、丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)和过氧化物酶(POD)活性均显著下降，其原因有待进一步研究。0.001-200 mg/L剂量组均显著降低轮虫幼虫的游泳线速度，100-200 mg/L剂量组显著抑制成虫的游泳线速度。这些变化会减少藻类食物的过滤，并可以解释存活和繁殖减少的原因。总体而言，溴酸盐对轮虫可能没有急性毒性，但对轮虫的行为仍有潜在的不利影响，进而可能影响水生态系统的群落结构。
The baking industries and disinfection of tap water released a considerable amount of bromate into surface water, which has been reported as a carcinogenic compound to mammals. Rotifers play an important role in freshwater ecosystems and are model organisms to assess environmental contamination. In the present study, the effects of different concentrations (0.001, 0.01, 0.1, 1, 10, 100 and 200 mg/L) of bromate on the life-table and population growth parameters were investigated in the rotifer Brachionus calyciflorus. The results showed that the 24-h LC50 of bromate to B. calyciflorus was 365.29 mg/L (95%Cl: 290.37-480.24). Treatments with 0.01, 10 and 200 mg/L bromate shorten the reproductive period. High levels of bromate (100 and 200 mg/L) significantly decreased net reproductive rate, intrinsic rate of population increase, life span, mictic rate of B. calyciflorus. To investigate the underlying mechanisms, swimming speed and antioxidative biomarkers were compared between bromate treatments and the control. The results showed that glutathione (GSH) and malondialdehyde (MDA) contents, total superoxide dismutase (T-SOD) and peroxidase (POD) activities decreased significantly in response to bromate exposure and the reasons required further investigation. Treatments with 0.001-200 mg/L bromate all significantly reduced swimming linear speed to rotifer larvae and treatments with 100-200 mg/L bromate significantly suppressed the swimming linear speed of adult rotifer. These changes would reduce filtration of algal food and could explain the decreased survival and reproduction. Overall, bromate may not show acute toxicity to rotifers, but still have potential adverse effects on rotifer behavior, which may then influence the community structure in aquatic ecosystems.