Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study.
Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study.
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DOI：
10.1186/gb-2007-8-12-r259
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Partensky F
中科院分区：
文献类型：
--
作者：
Six C;Thomas JC;Garczarek L;Ostrowski M;Dufresne A;Blot N;Scanlan DJ;Partensky F
通过比较聚球藻的基因组，确定了产生藻胆蛋白所需的候选基因，藻胆蛋白是被称为藻胆体的捕光天线复合体的一部分。系统发育分析表明，藻胆体核心与核心基因组一起进化，而杆状结构独立进化。海洋聚球藻因其独特的鲜艳颜色(从蓝绿色到橙色)而被称为藻胆体的大型外在天线复合体，由中央别藻蓝蛋白核心和各种藻胆蛋白组成的杆组成。根据藻体中发现的主要藻胆蛋白(藻蓝蛋白、藻红蛋白I或藻红蛋白II)，可以定义三种主要的色素类型。在同时含有藻红蛋白I和II的菌株中，可以根据与这些藻胆蛋白结合的两个发色团的比率来区分四个亚型。最近，11株海洋聚球藻的基因组得以获得，每个色素类型或亚型有一到四个菌株，这使得对参与藻胆体新陈代谢的基因进行了前所未有的比较基因组学研究。通过仔细比较聚球藻的基因组，我们已经找回了每种色素类型中合成藻胆蛋白可能需要的候选基因。这包括连接多肽、藻胆蛋白裂解酶和一些新的功能未知的基因。有趣的是，属于特定色素类型的菌株具有类似的藻胆体基因互补和组织，与核心基因组系统发育无关(如使用连接的核糖体蛋白进行评估)。基于连接的别藻蓝蛋白蛋白序列的系统发育树与后者一致，而基于藻蓝蛋白和藻红蛋白的系统发育树明显不同，并与聚球藻的色素类型相匹配。我们的结论是，藻胆体核心可能与核心基因组一起进化，而杆状突变体必须是独立进化的，可能是通过病毒或自然转化在聚球藻之间横向转移藻胆体杆状基因或基因簇，从而快速适应各种光生态位。
By comparing Synechococcus genomes, candidate genes required for the production of phycobiliproteins, which are part of the light-harvesting antenna complexes called phycobilisomes, were identified. Phylogenetic analyses suggest that the phycobilisome core evolved together with the core genome, whereas rods evolved independently. Marine Synechococcus owe their specific vivid color (ranging from blue-green to orange) to their large extrinsic antenna complexes called phycobilisomes, comprising a central allophycocyanin core and rods of variable phycobiliprotein composition. Three major pigment types can be defined depending on the major phycobiliprotein found in the rods (phycocyanin, phycoerythrin I or phycoerythrin II). Among strains containing both phycoerythrins I and II, four subtypes can be distinguished based on the ratio of the two chromophores bound to these phycobiliproteins. Genomes of eleven marine Synechococcus strains recently became available with one to four strains per pigment type or subtype, allowing an unprecedented comparative genomics study of genes involved in phycobilisome metabolism. By carefully comparing the Synechococcus genomes, we have retrieved candidate genes potentially required for the synthesis of phycobiliproteins in each pigment type. This includes linker polypeptides, phycobilin lyases and a number of novel genes of uncharacterized function. Interestingly, strains belonging to a given pigment type have similar phycobilisome gene complements and organization, independent of the core genome phylogeny (as assessed using concatenated ribosomal proteins). While phylogenetic trees based on concatenated allophycocyanin protein sequences are congruent with the latter, those based on phycocyanin and phycoerythrin notably differ and match the Synechococcus pigment types. We conclude that the phycobilisome core has likely evolved together with the core genome, while rods must have evolved independently, possibly by lateral transfer of phycobilisome rod genes or gene clusters between Synechococcus strains, either via viruses or by natural transformation, allowing rapid adaptation to a variety of light niches.
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影响因子：
4.4
作者：
Fuller, NJ;Marie, D;Scanlan, DJ
通讯作者：
Scanlan, DJ
影响因子：
5.1
作者：
BHALERAO, RP;LIND, LK;GUSTAFSSON, P
通讯作者：
GUSTAFSSON, P
影响因子：
3.2
作者：
FEDERSPIEL, NA;GROSSMAN, AR
通讯作者：
GROSSMAN, AR
影响因子：
4.4
作者：
Chen, F;Wang, K;Wommack, KE
通讯作者：
Wommack, KE
影响因子：
2.9
作者：
Everroad, R. Craig;Wood, A. Michelle
通讯作者：
Wood, A. Michelle