BacFlash signals acid-resistance gene expression in bacteria
BacFlash signals acid-resistance gene expression in bacteria
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BacFlash 发出细菌抗酸基因表达的信号
DOI：
10.1038/s41422-020-00431-3
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发表时间：
2020-11
期刊：
影响因子：
44.1
通讯作者：
Wang Xianhua
中科院分区：
文献类型：
--
作者：
Wu Di;Qi Wenfeng;Nie Wei;Lu Zhengyuan;Ye Yongxin;Li Jinghang;Sun Tao;Zhu Yufei;Cheng Heping;Wang Xianhua
Intracellular pH (pHi) homeostasis is crucial for cellular functions and signal transduction across all kingdoms of life. In particular, bacterial pHihomeostasis is important for physiology, ecology, and pathogenesis. Here we report an exquisite bacterial acid-resistance (AR) mechanism in which proton leak elicits a pre-emptive AR response. A single bacterial cell undergoes quantal electrochemical excitation, termed “BacFlash”, which consists of membrane depolarization, transient pHirise, and bursting production of reactive oxygen species. BacFlash ignition is dictated by acid stress in the form of proton leak across the plasma membrane and the rate of BacFlash occurrence is reversely correlated with the pHibuffering capacity. Through genome-wide screening, we further identify the ATP synthase Focomplex subunit a as the putative proton sensor for BacFlash biogenesis. Importantly, persistent BacFlash hyperactivity activates transcription of a panel of key AR genes and predisposes the cells to survive imminent extreme acid stress. These findings demonstrate a prototypical coupling between electrochemical excitation and nucleoid gene expression in prokaryotes.
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DOI：
10.1016/s0021-9258(17)39639-4
发表时间：
1985-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Jayakumar;W. Epstein;E. M. Barnes
通讯作者：
A. Jayakumar;W. Epstein;E. M. Barnes
影响因子：
82.9
作者：
Breckwoldt, Michael;Pfister, Franz M. J.;Misgeld, Thomas
通讯作者：
Misgeld, Thomas
DOI：
10.1128/genomea.01038-14
发表时间：
2014-10-16
期刊：
Genome announcements
影响因子：
--
作者：
Grenier F;Matteau D;Baby V;Rodrigue S
通讯作者：
Rodrigue S
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影响因子：
6.6
作者：
Wang W;Gong G;Wang X;Wei-LaPierre L;Cheng H;Dirksen R;Sheu SS
通讯作者：
Sheu SS
影响因子：
2.8
作者：
D. C. Yoch;Zhaolei Zhang;D. L. Claybrook
通讯作者：
D. C. Yoch;Zhaolei Zhang;D. L. Claybrook