THE HUMAN SPERM PROTEIN PH-20 HAS HYALURONIDASE ACTIVITY
THE HUMAN SPERM PROTEIN PH-20 HAS HYALURONIDASE ACTIVITY
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DOI：
10.1016/0014-5793(93)80873-s
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发表时间：
1993-12-28
期刊：
影响因子：
3.5
通讯作者：
KETTER, S
中科院分区：
文献类型：
--
作者：
GMACHL, M;SAGAN, S;KETTER, S
使用单克隆抗体表征豚鼠精子膜上存在的PH-20蛋白[（1991）J. Cell Biol.111，2939-2949]。我们已经从睾丸文库中分离出了编码人PH-20蛋白的cDNA。使用牛痘病毒表达系统在RK 13细胞中表达该cDNA。表达人PH-20蛋白的细胞具有透明质酸酶活性。用PI-PLC处理将透明质酸酶释放到培养基中，伴随着酶活性的大幅增加。这些结果证明人PH-20蛋白具有透明质酸酶活性。
The PH-20 protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [(1991) J. Cell Biol. 111, 2939-2949]. We have isolated the cDNA encoding the human PH-20 protein from a testis library. This cDNA was expressed in RK 13 cells using a vaccinia virus expression system. Cells expressing the human PH-20 protein possess hyaluronidase activity. Treatment with PI-PLC releases the hyaluronidase into the the medium with a concomitant large increase in enzymatic activity. These results demonstrate that the human PH-20 protein has hyaluronidase activity.