Prolactin receptor expression in the developing human prostate and in hyperplastic, dysplastic, and neoplastic lesions
Prolactin receptor expression in the developing human prostate and in hyperplastic, dysplastic, and neoplastic lesions
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DOI：
10.1016/s0002-9440(10)65333-3
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发表时间：
1999-03-01
影响因子：
6
通讯作者：
Ho, SM
中科院分区：
文献类型：
--
作者：
Leav, I;Merk, FB;Ho, SM
原位杂交和免疫组化被用来定位和比较的人催乳素受体的长型在胎儿，青春期前，和成人前列腺的表达。然后将结果与增生、异型增生和肿瘤性病变进行比较。受体信息和蛋白主要定位于胎儿、新生儿、青春期前和正常成人前列腺的上皮细胞中。在增生性病变的受体的表达是不变的，相对于正常的上皮细胞。无论级别，明显增强的受体表达是明显的发育不良病变。在Gleason分级较低的癌中，受体信号在信息和蛋白水平上的强度接近于正常前列腺上皮中发现的强度。然而，在较高分级的癌中的病灶中，受体表达似乎减少。我们的研究结果表明，催乳素作用在人前列腺的发育和维持中起作用，并且还可能参与前列腺的早期肿瘤转化。高级别肿瘤中受体表达的减少可能反映了不再对肽激素有反应的细胞群的出现。
In situ hybridization and immunohistochemistry were used to localize and compare the expression of the long form of the human prolactin receptor in fetal, prepubertal, and adult prostate. Results were then compared with hyperplastic, dysplastic, and neoplastic lesions, Both receptor message and protein were predominately localized in epithelial cells of the fetal, neonatal, prepubertal, and normal adult prostate. In hyperplastic lesions the expression of the receptor was unchanged with respect to normal epithelial cells. Irrespective of grade, markedly enhanced expression of the receptor was evident in dysplastic lesions. In lower Gleason grade carcinomas the intensity of receptor signal at the message and protein levels approximated that found in normal prostatic epithelium However, In foci within higher grade cancers, receptor expression appeared diminished. Results from our study suggest that prolactin action plays a role in the development and maintenance of the human prostate and may also participate In early neoplastic transformation of the gland. Diminution of receptor expression in high grade neoplasms could reflect the emergence of a population of cells that are no longer responsive to the peptide hormone.