Reversed-phase ion-pair chromatography coupled to electrospray ionisation mass spectrometry by on-line removal of the counter-ions
Reversed-phase ion-pair chromatography coupled to electrospray ionisation mass spectrometry by on-line removal of the counter-ions
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DOI：
10.1016/s0021-9673(99)00526-9
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发表时间：
1999-08-27
影响因子：
4.1
通讯作者：
Långström, B
中科院分区：
文献类型：
--
作者：
Forngren, BH;Samskog, J;Långström, B
使用强阴离子交换剂作为捕获柱进行离子对色谱与电喷雾电离质谱的在线耦合。在反相液相色谱中，离子对试剂用于保留低分子量的极性分析物远离溶剂前沿。捕获柱能够在检测之前从流动相中去除非挥发性抗衡离子，以便可以在有利的电离条件下进行电喷雾过程，并且不会污染界面。研究了以 1-辛烷磺酸和十二烷基硫酸钠作为离子对试剂的捕获过程的效率。使用这种在线捕获方法，生物蝶呤和胍可以以 k'>2 保留，并通过电喷雾质谱法检测，信号稳定。 (C) 1999 Elsevier Science B.V. 保留所有权利。
A strong anion-exchanger was used as a trapping column to perform on-line coupling of ion-pair chromatography with electrospray ionisation mass spectrometry. Ion-pairing reagents were used to retain polar analytes of low molecular mass away from the solvent front in reversed-phase LC. The trapping column enabled removal of the non-volatile counter-ions from the mobile phase prior to detection, so that the electrospray process could be performed with favourable ionisation conditions and without contamination of the interface. The efficiency of the trapping process was studied for 1-octanesulfonic acid and sodium dodecyl sulfate as ion-pairing reagents. Using this on-line trapping method, biopterin and guanidine could be retained with a k'>2 and detected by electrospray mass spectrometry with a stable signal. (C) 1999 Elsevier Science B.V. All rights reserved.