PURIFICATION AND BIOPHYSICAL CHARACTERIZATION OF HEPATITIS-B ANTIGEN
PURIFICATION AND BIOPHYSICAL CHARACTERIZATION OF HEPATITIS-B ANTIGEN
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DOI：
10.1172/jci107284
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
TILLES, JG
中科院分区：
文献类型：
--
作者：
KIM, CY;TILLES, JG
用胃蛋白酶消化和平衡沉降法从血清中纯化出B型肝炎抗原（HB Ag）。在纯化前后测定了沉降系数、密度、粒度和电泳迁移率，发现其未发生变化。此外，纯化后的乙肝抗原的扩散常数和分子量也得到了证实。由于所发现的生物物理特性与任何已知病毒的生物物理特性都不一致，因此认为天然乙肝抗原几乎完全由不完整的病毒组成。
Hepatitis B antigen (HB Ag) has been purified from serum by pepsin digestion and equilibrium sedimentation. The sedimentation coefficient, density, particle size, and electrophoretic mobility have been determined before and after purification and found to be unaltered. In addition, the diffusion constant and molecular weight of purified HB Ag have been documented.Because the biophysical characteristics found are not consistant with those of any known virus, it is believed that native HB Ag is made up almost entirely of incomplete virus.Images