Associations between properties linked with persistence in a collection of Staphylococcus aureus isolates from bovine mastitis
Associations between properties linked with persistence in a collection of Staphylococcus aureus isolates from bovine mastitis
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DOI：
10.1016/j.vetmic.2013.12.010
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发表时间：
2014-02-21
影响因子：
3.3
通讯作者：
Ote, Isabelle
中科院分区：
文献类型：
--
作者：
Bardiau, Marjorie;Detilleux, Johann;Ote, Isabelle
金黄色葡萄球菌是世界公认的引起人和动物多种严重疾病的病原体，是临床和亚临床牛乳房炎最重要的病原体之一。本研究的目的是调查和关联从患有乳房炎的奶牛的牛奶中分离的金黄色葡萄球菌可能与持续性乳房炎有关的特性：(I)通过特异性ELISA检测衣壳抗原(CP5或CP8)的表达；(Ii)通过入侵MAC-T细胞在细胞内存活；以及(Iii)通过分光光度分析在TSBglc中生长后产生生物膜。结果表明：(1)表达衣壳抗原的菌株在Cap8-中的比例高于Cap5阳性菌株；(2)衣壳形态与细胞内存活率和生物被膜产量相关；(3)衣壳形态、生物被膜产量和细胞内存活率仅与两个agr-群相关。统计和聚类分析使我们能够建立与体内持久性相关的不同特征。事实上，属于AGR组II的分离株，表达囊膜抗原CP8，并表现出较低的细胞内存活率，可能更适合细胞外生态位。相反，不表达任何衣壳抗原(CP5或CP8)但表现出高细胞内存活率的属于AGR组I的分离株可能更适合细胞内生态位。(C)2013爱思唯尔B.V.保留所有权利。
Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals, and is one of the most important etiological agents of clinical and subclinical bovine mastitis. The aim of the present study was to investigate and correlate properties, that may be associated with persistent mastitis, of S. aureus strains isolated from milk of cows suffering from mastitis: (i) expression of capsular antigens (CP5 or CP8) by specific ELISA; (ii) intracellular survival by invasion of MAC-T cells; and (iii) biofilm production by spectrophotometry analysis after growth in TSBglc. The results showed that (i) the proportion of strains expressing capsular antigen was higher in cap8- than in cap5-positive isolates; (ii) a correlation was observed between the capsular profile and the intracellular survival as well as the biofilm production; and (iii) the capsular profile, biofilm production and intracellular survival were associated with only two agr-groups. Statistical and clustering analysis allowed us to establish different profiles that could be associated with in vivo persistence. Indeed, isolates belonging to agr group II, expressing the capsular antigen CP8 and showing low intracellular survival are probably better adapted to an extracellular niche. Conversely, isolates belonging to agr group I that do not express any capsular antigen (CP5 or CP8) but show high intracellular survival are probably better adapted to an intracellular niche. (C) 2013 Elsevier B.V. All rights reserved.