Effect of methyl tert-butyl ether on adipogenesis and glucose metabolism in vitro and in vivo
Effect of methyl tert-butyl ether on adipogenesis and glucose metabolism in vitro and in vivo
复制标题
甲基叔丁基醚对体内外脂肪生成和葡萄糖代谢的影响
DOI：
10.1016/j.jes.2019.06.015
复制
发表时间：
2019
影响因子：
6.9
通讯作者：
Du Yuguo
中科院分区：
文献类型：
--
作者：
Tang Yue;Ren Qidong;Wen Qing;Yu Caixia;Xie Xinni;Hu Qing;Du Yuguo
甲基叔丁基醚（MTBE）作为一种广泛使用的汽油添加剂，被怀疑具有环境毒性。甲基叔丁基醚主要在脂肪组织中积累，但其对肥胖或肥胖相关代谢紊乱的影响尚未得到很好的了解。因此，我们用3 T3-L1细胞系和C57 BL/6 J小鼠模型研究了MTBE对脂肪功能和相关代谢过程的影响。结果发现，环境相关浓度（100 μmol/L）的MTBE可显著诱导前脂肪细胞分化，并干扰胰岛素刺激的成熟脂肪细胞葡萄糖摄取。雄性小鼠体内观察显示，在14周内，与MTBE处理相比，内脏白色脂肪组织（vWAT）扩张和细胞大小增加呈正相关。葡萄糖耐量和胰岛素敏感性试验结果表明，1000 μg/（kg·d）剂量的MTBE对大鼠的全身葡萄糖代谢产生了显著的影响，这可能与阿克曼氏菌、梭菌和巨单胞菌属水平上肠道菌群的改变有关。总之，我们的研究表征了MTBE对体外和体内脂肪组织功能和葡萄糖稳态的影响，并揭示了相关肠道微生物群可能调节葡萄糖代谢的全身性疾病。
Methyltert-butyl ether (MTBE), as a widely used gasoline additive, is suspected of being environmentally toxic. MTBE accumulates mainly in adipose tissue, but its effect on obesity or obesity-related metabolic disorders has not been well understood yet. Therefore, we examined the effect of MTBE on the adipose function and the related metabolic processes with both 3T3-L1 cell line and C57BL/6J mice model. We found that exposure to MTBE at the environmental relevant concentration (100 μmol/L) could significantly induce differentiation of preadipocyte and disturb insulin-stimulated glucose uptake of mature adipocyte. Thein vivoobservation in male mice showed a positive correlation of visceral white adipose tissue (vWAT) expansion and cell size increase with MTBE treatment in 14 weeks. Glucose tolerance and insulin sensitivity tests demonstrated that MTBE at 1000 μg/(kg·day) disturbed the systemic glucose metabolism in a gender-specific manner, which might be partly attributed to the alterations of gut microbiota community at genus level with respect toAkkermansia, ClostridiumXlVb,andMegamonas. In summary, our study characterized the effect of MTBE on adipose tissue function and glucose homeostasisin vitroandin vivo, and revealed that systemic disorders of the glucose metabolism might be modulated by the related gut microbiota.