SINI MODULATES THE ACTIVITY OF SINR, A DEVELOPMENTAL SWITCH PROTEIN OF BACILLUS-SUBTILIS, BY PROTEIN PROTEIN-INTERACTION
SINI MODULATES THE ACTIVITY OF SINR, A DEVELOPMENTAL SWITCH PROTEIN OF BACILLUS-SUBTILIS, BY PROTEIN PROTEIN-INTERACTION
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DOI：
10.1101/gad.7.1.139
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发表时间：
1993-01-01
影响因子：
10.5
通讯作者：
SMITH, I
中科院分区：
文献类型：
--
作者：
BAI, U;MANDICMULEC, I;SMITH, I
SINR是一种111个氨基酸的DNA结合蛋白，对枯草芽孢杆菌的几个后期生长过程具有多效性调节作用。它起着发育开关的作用，正向调节能力和运动性基因，抑制apre和II期产孢子基因。它由两个基因操纵子中的第二个基因编码，但先前的结果也表明，这两个基因受到不同的调控。我们在讨论中发现，该操纵子的第一个开放阅读框架(ORF)SINI的产物干扰了SINR的功能。体内实验已经证明，过表达SINI会导致在sinR零突变的细胞中观察到的表型。SINI的染色体框内缺失导致与较高水平的SINR相关的表型。因此，SINI是SINR的拮抗剂。体外实验表明，这两种蛋白质之间的相互作用是直接的。SINI阻止SINR与APRE上的靶序列结合，这两种蛋白形成的复合体可以与SINR或SINI的抗体免疫共沉淀。
SinR, a 111-amino-acid DNA-binding protein, is a pleiotropic regulator of several late growth processes in Bacillus subtilis. It acts as a developmental switch, positively regulating genes for competence and motility and repressing aprE and stage II sporulation genes. It is encoded by the second gene in a two gene operon, but previous results have also indicated that these two genes are differently regulated. We show in this discussion that the product of sinI, the first open reading frame (ORF) of this operon, interferes with the function of SinR. In vivo experiments have demonstrated that overexpression of sinI results in phenotypes that are observed in cells with a null mutation of sinR. A chromosomal in-frame deletion of sinI gives rise to a phenotype associated with higher levels of SinR. Thus, SinI acts as an antagonist to SinR. In vitro experiments have shown that the interaction between these two proteins is a direct one. SinI prevents SinR from binding to its target sequence on aprE, and the two proteins form a complex that can be immunoprecipitated with antibodies to either SinR or SinI.