The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and Cucumber mosaic virus resistance
The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and Cucumber mosaic virus resistance
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DOI：
10.1007/s10526-010-9306-x
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发表时间：
2011-02
期刊：
影响因子：
2.5
通讯作者：
Shuai Wang;Huijun Wu;J. Zhan;Yanfei Xia;Shengfeng Gao;Weiduo Wang;P. Xue;Xuewen Gao
中科院分区：
文献类型：
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作者：
Shuai Wang;Huijun Wu;J. Zhan;Yanfei Xia;Shengfeng Gao;Weiduo Wang;P. Xue;Xuewen Gao
枯草芽孢杆菌以其对多种植物病原菌的生物防治活性和促进植物生长的作用而闻名。HpaGXooc来自水稻病原细菌水稻黄单胞菌（Xanthomonasoryzaepv.oryzicola），是harpin蛋白家族的成员。已知它在非宿主植物中引起过敏性细胞死亡，从而诱导植物的抗病性和抗虫性并增强植物生长。在我们之前的实验中，我们构建了一个基因工程菌株-B。枯草杆菌OKBHF-通过引入编码HpaGXooctoB的基因。为了联合收割机结合HpaGXooc和野生型PGPR在提高植物生长速率和生物防治中的作用，在这项研究中，我们评估了使用B。枯草杆菌OKBHF。温室试验结果表明，接种后28 d，OKBHF处理对黄瓜株高、鲜重、花果数有显著的增加作用，并能明显降低黄瓜花叶病毒（CMV）的发病程度。随后的逆转录-聚合酶链反应分析揭示了HpaGXoocandB的分子机制。枯草杆菌在番茄植株中的表达，表明它们在诱导调节植物细胞生长的三个扩展蛋白基因LeEXP 2、LeEXP 5和LeEXP 18以及激活广泛的PR基因和一个防御基因PR-1a的表达的两个防御相关基因Pti 4和Pti 6的增强表达中具有协同作用。
Bacillussubtilisis well known for its biocontrol activity against several plant pathogens and for its role in promoting plant growth. HpaGXooc, from rice pathogenic bacteriumXanthomonasoryzaepv.oryzicola, is a member of the harpin group of proteins. It is known to elicit hypersensitive cell death in non-host plants, thereby inducing disease and insect resistance in the plants and enhancing plant growth. In our previous experiment, we constructed a genetically engineered strain—B. subtilisOKBHF—through the introduction of the gene encoding HpaGXoocintoB. subtilisOKB105 in order to combine the effects of HpaGXoocand wild-type PGPR in improving the plant growth rate and for biological control. In this study, we evaluated the use of treating the tomato plant withB. subtilisOKBHF. The results of greenhouse experiments demonstrated that OKBHF treatment had a significant effect on increasing the height, fresh weight, and flower and fruit number and obviously lowered the disease severity of Cucumber mosaic virus (CMV) infection at 28 days postinoculation (dpi). Subsequent reverse transcription-polymerase chain reaction analysis revealed the molecular mechanisms of HpaGXoocandB. subtilisin the tomato plant, suggesting their synergistic roles in inducing enhanced expression of three expansin genesLeEXP2,LeEXP5, andLeEXP18, which regulate plant cell growth, and two defense-related genesPti4andPti6, which activate the expression of a wide array ofPRgenes and one defense gene,PR-1a.